Science and engineering rely on the accumulation and dissemination of knowledge to make
discoveries and create new designs. Discovery‐driven genome research rests on …

The genomic revolution is fully upon us in 2006 and the pace of discovery is set to
accelerate with the emergence of ultra-high-throughput sequencing technologies. Our …

Background De novo genome sequencing of previously uncharacterized microorganisms
has the potential to open up new frontiers in microbial genomics by providing insight into …

Innovations in sequencing technologies have allowed biologists to make incredible
advances in understanding biological systems. As experience grows, researchers …

Long read sequencing technologies now allow routine highly contiguous assembly of
bacterial genomes. However, because of the lower accuracy of some long read data, it is …

Motivation: A large and rapidly growing number of bacterial organisms have been
sequenced by the newest sequencing technologies. Cheaper and faster sequencing …

Illumina sequencing allows rapid, cheap and accurate whole genome bacterial analyses,
but short reads (< 300 bp) do not usually enable complete genome assembly. Long-read …

Highlights•We describe three long-read sequencing technologies and their
characteristics.•We describe available algorithms for long-read assembly and their …

Whole genome sequencing has increasingly become the essential method for studying the
genetic mechanisms of antimicrobial resistance and for surveillance of drug-resistant …

Motivation: Assemblies of next-generation sequencing (NGS) data, although accurate, still
contain a substantial number of errors that need to be corrected after the assembly process …