Redox stress reshapes carbon fluxes of Pseudomonas putida for cytosolic glucose oxidation and NADPH generation

PI Nikel, T Fuhrer, M Chavarría, A Sánchez-Pascuala… - BioRxiv, 2020 - biorxiv.org
The soil bacterium and metabolic engineering platform Pseudomonas putida tolerates high
levels of endogenous and exogenous oxidative stress, yet the ultimate reason of such …

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

PI Nikel, T Fuhrer, M Chavarría… - The ISME …, 2021 - academic.oup.com
As a frequent inhabitant of sites polluted with toxic chemicals, the soil bacterium and plant-
root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous …

Response of Pseudomonas putida KT2440 to increased NADH and ATP demand

BE Ebert, F Kurth, M Grund, LM Blank… - Applied and …, 2011 - Am Soc Microbiol
Adenosine phosphate and NAD cofactors play a vital role in the operation of cell
metabolism, and their levels and ratios are carefully regulated in tight ranges. Perturbations …

Multi-omics analysis unravels a segregated metabolic flux network that tunes co-utilization of sugar and aromatic carbons in Pseudomonas putida

MA Kukurugya, CM Mendonca, M Solhtalab… - Journal of Biological …, 2019 - ASBMB
Pseudomonas species thrive in different nutritional environments and can catabolize
divergent carbon substrates. These capabilities have important implications for the role of …

[HTML][HTML] Reserve flux capacity in the pentose phosphate pathway enables Escherichia coli's rapid response to oxidative stress

D Christodoulou, H Link, T Fuhrer, K Kochanowski… - Cell systems, 2018 - cell.com
To counteract oxidative stress and reactive oxygen species (ROS), bacteria evolved various
mechanisms, primarily reducing ROS through antioxidant systems that utilize cofactor …

Metabolic response of Pseudomonas putida to increased NADH regeneration rates

S Zobel, J Kuepper, B Ebert, N Wierckx… - Engineering in Life …, 2017 - Wiley Online Library
Pseudomonas putida efficiently utilizes many different carbon sources without the formation
of byproducts even under conditions of stress. This implies a high degree of flexibility to …

Pseudomonas putida KT2440 is naturally endowed to withstand industrial‐scale stress conditions

A Ankenbauer, RA Schäfer, SC Viegas… - Microbial …, 2020 - Wiley Online Library
Pseudomonas putida is recognized as a very promising strain for industrial application due
to its high redox capacity and frequently observed tolerance towards organic solvents. In this …

Synthetic control of metabolic states in Pseudomonas putida by tuning polyhydroxyalkanoate Cycle

MT Manoli, J Nogales, A Prieto - Mbio, 2022 - Am Soc Microbiol
Polyhydroxyalkanoates (PHAs) are polyesters produced by numerous microorganisms for
energy and carbon storage. Simultaneous synthesis and degradation of PHA drives a …

[HTML][HTML] Pseudomonas putida as a functional chassis for industrial biocatalysis: from native biochemistry to trans-metabolism

PI Nikel, V de Lorenzo - Metabolic engineering, 2018 - Elsevier
The itinerary followed by Pseudomonas putida from being a soil-dweller and plant colonizer
bacterium to become a flexible and engineer-able platform for metabolic engineering stems …

Aspartate metabolism and pyruvate homeostasis triggered by oxidative stress in Pseudomonas fluorescens: a functional metabolomic study

A Alhasawi, M Leblanc, ND Appanna, C Auger… - Metabolomics, 2015 - Springer
There is mounting evidence that metabolic reprogramming is critical for the survival of
organisms exposed to changing and stressed environments. Using the soil microbe …