Isothermal titration calorimetry as a complementary method for investigating nanoparticle–protein interactions

D Prozeller, S Morsbach, K Landfester - Nanoscale, 2019 - pubs.rsc.org
Isothermal titration calorimetry (ITC) is a complementary technique that can be used for
investigations of protein adsorption on nanomaterials, as it quantifies the thermodynamic …

Biomolecule–nanoparticle interactions: Elucidation of the thermodynamics by isothermal titration calorimetry

R Huang, BLT Lau - Biochimica et Biophysica Acta (BBA)-General Subjects, 2016 - Elsevier
Abstract Background Nanomaterials (NMs) are often exposed to a broad range of
biomolecules of different abundances. Biomolecule sorption driven by various interfacial …

Application of isothermal titration calorimetry for characterizing thermodynamic parameters of biomolecular interactions: peptide self-assembly and protein adsorption …

M Kabiri, LD Unsworth - Biomacromolecules, 2014 - ACS Publications
The complex nature of macromolecular interactions usually makes it very hard to identify the
molecular-level mechanisms that ultimately dictate the result of these interactions. This is …

Probing the interaction of lysozyme with ciprofloxacin in the presence of different-sized Ag nano-particles by multispectroscopic techniques and isothermal titration …

F Pasban Ziyarat, A Asoodeh… - Journal of …, 2014 - Taylor & Francis
The binding of ciprofloxacin to lysozyme in the presence of three Ag nano-particles of
varying sizes was for the first time investigated by multispectroscopic and isothermal titration …

Biomimetic interactions of proteins with functionalized nanoparticles: a thermodynamic study

M De, CC You, S Srivastava… - Journal of the American …, 2007 - ACS Publications
Gold nanoparticles (NPs) functionalized with l-amino acid-terminated monolayers provide
an effective platform for the recognition of protein surfaces. Isothermal titration calorimetry …

Contrasting effect of gold nanoparticles and nanorods with different surface modifications on the structure and activity of bovine serum albumin

S Chakraborty, P Joshi, V Shanker, ZA Ansari… - Langmuir, 2011 - ACS Publications
Nanoparticles exposed to biofluids become coated with proteins, thus making protein–
nanoparticle interactions of particular interest. The consequence on protein conformation …

Nanoparticle and protein corona

M Rahman, S Laurent, N Tawil, LH Yahia… - … Interactions: The Bio …, 2013 - Springer
Nanoparticles and other nanomaterials are increasingly considered for use in biomedical
applications such as imaging, drug delivery, and hyperthermic therapies. Thus …

Probing the binding affinity of plasma proteins adsorbed on Au nanoparticles

X Zhang, J Zhang, F Zhang, S Yu - Nanoscale, 2017 - pubs.rsc.org
Nanoparticle (NP) surfaces are modified immediately by the adsorption of proteins when
exposed to human blood, leading to the formation of a protein corona. The adsorption of …

Isothermal titration calorimetry studies on the binding of amino acids to gold nanoparticles

H Joshi, PS Shirude, V Bansal… - The Journal of …, 2004 - ACS Publications
Isothermal titration calorimetry (ITC) is a powerful and highly sensitive technique commonly
used to study interactions between biomolecules in dilute aqueous solutions, both from …

Hard and soft protein corona of nanomaterials: Analysis and relevance

R García-Álvarez, M Vallet-Regí - Nanomaterials, 2021 - mdpi.com
Upon contact with a biological milieu, nanomaterials tend to interact with biomolecules
present in the media, especially proteins, leading to the formation of the so-called “protein …