Despite the many approaches to study differential splicing from RNA-seq, many challenges
remain unsolved, including computing capacity and sequencing depth requirements. Here …

Alternative splicing (AS) contributes significantly to RNA and protein variability yet its role in
defining cellular diversity is not fully understood. While Smart-seq2 offers enhanced …

The vast majority of multi-exon genes in higher eukaryotes are alternatively spliced and
changes in alternative splicing (AS) can impact gene function or cause disease. High …

Single-cell sequencing technologies have revolutionised the life sciences and biomedical
research. Single-cell sequencing provides high-resolution data on cell heterogeneity …

Alternative splicing was found to be a common phenomenon after the advent of whole
transcriptome analyses or next generation sequencing. Over 90% of human genes were …

Alternative splicing of pre-mRNA is a key mechanism for increasing the complexity of
proteins in humans, causing a diversity of expression of transcriptomes and proteomes in a …

Alternative gene splicing is a common phenomenon in which a single gene gives rise to
multiple transcript isoforms. The process is strictly guided and involves a multitude of …

Most genes are processed by alternative splicing for gene expression, resulting in the
complexity of the transcriptome in eukaryotes. It allows a limited number of genes to encode …

Alternative splicing is a substantial contributor to the high complexity of transcriptomes of
multicellular eukaryotes. In this Review, we discuss the accumulated evidence that most of …

Alternative splicing alterations have been widely related to several human diseases
revealing the importance of their study for the success of translational medicine. Differential …