Microtubules (MTs) are the most rigid elements of the cytoskeleton with in vitro persistence
lengths (L p) in the range of 1–6 mm. In cellular environments, however, MTs often appear …

The microtubule cytoskeleton is involved in many vital cellular processes. Microtubules act
as tracks for molecular motors, and their polymerization and depolymerization can be …

Traditionally, structures of cytoskeletal components have been studied ex situ, that is, with
biochemically purified materials. There are compelling reasons to develop approaches to …

Microtubules and filamentous (F-) actin engage in complex interactions to drive many
cellular processes from subcellular organization to cell division and migration. This is …

Cryo-EM is ideally suited for the study of cytoskeleton polymers and their interaction with
cellular partners. Our understanding of microtubule (MT) structure and interactions has …

Microtubules are tubular polymers with essential roles in numerous cellular activities.
Structures of microtubules have been captured at increasing resolution by cryo-EM …

The cytoskeleton is involved in numerous cellular processes such as migration, division, and
contraction and provides the tracks for transport driven by molecular motors. Therefore, it is …

Highlights•Technical progress leads to high-resolution cryo-EM structures of microtubules
(MTs).•Atomic details of MT-binding proteins and antimitotic drugs bound to …

The development of cryo-electron microscopy (cryo-EM) allowed microtubules to be
captured in their solution-like state, enabling decades of insight into their dynamic …

A precise quantitative description of the ultrastructural characteristics underlying biological
mechanisms is often key to their understanding. This is particularly true for dynamic extra …