Bioprospecting for thermozymes and characterization of a novel lipolytic thermozyme belonging to the SGNH/GDSL family of hydrolases

JJ Escuder-Rodríguez, ME DeCastro… - International Journal of …, 2022 - mdpi.com
Functional screenings were conducted on two metagenomic libraries from hot springs in
order to find novel thermozymes with potential biotechnological applications. These …

[HTML][HTML] Heterologous expression and characterization of glycoside hydrolase with its potential applications in hyperthermic environment

M Mustafa, L Ali, W Islam, A Noman, C Zhou… - Saudi Journal of …, 2022 - Elsevier
With the progressive focus on renewable energy via biofuels production from lignocellulosic
biomass, cellulases are the key enzymes that play a fundamental role in this regard. This …

A multi-omic screening approach for the discovery of thermoactive glycoside hydrolases

P Busch, M Suleiman, C Schäfers, G Antranikian - Extremophiles, 2021 - Springer
Next-generation sequencing and computational biology have facilitated the implementation
of new combinatorial screening approaches to discover novel enzymes of biotechnological …

Cloning and characterization of a novel cold-active glycoside hydrolase family 1 enzyme with β-glucosidase, β-fucosidase and β-galactosidase activities

A Wierzbicka-Woś, P Bartasun, H Cieśliński, J Kur - BMC biotechnology, 2013 - Springer
Background Cold-active enzymes, sourced from cold-adapted organisms, are characterized
by high catalytic efficiencies at low temperatures compared with their mesophilic …

Glycosyl hydrolases from hyperthermophilic microorganisms

MW Bauer, LE Driskill, RM Kelly - Current Opinion in Biotechnology, 1998 - Elsevier
Glycosyl hydrolases from hyperthermophiles are, thus far, the most widely studied enzyme
class from these organisms. Not only are there many biotechnological opportunities for …

Molecular cloning, expression, and characterization of four novel thermo-alkaliphilic enzymes retrieved from a metagenomic library

M Maruthamuthu, JD van Elsas - Biotechnology for biofuels, 2017 - Springer
Background Enzyme discovery is a promising approach to aid in the deconstruction of
recalcitrant plant biomass in an industrial process. Novel enzymes can be readily …

Discovering novel hydrolases from hot environments

R Wohlgemuth, J Littlechild, D Monti, K Schnorr… - Biotechnology …, 2018 - Elsevier
Novel hydrolases from hot and other extreme environments showing appropriate
performance and/or novel functionalities and new approaches for their systematic screening …

Thermodynamic properties of the β-glucosidase from Thermotoga maritima extend the upper limit of thermophilicity

MA Mehmood, I Shahid, K Hussain… - Protein and Peptide …, 2014 - ingentaconnect.com
Enzymes from thermophilic organisms are believed to be strong candidates for industrial
applications due to their ability to withstand temperature-induced enzyme inactivation. The …

Industrial applications of hyperthermophilic enzymes: a review

T de Miguel Bouzas, J Barros-Velázquez… - Protein and peptide …, 2006 - ingentaconnect.com
Over the past two decades, research scientists have been involved in the investigation of
thermophilic and hyperthermophilic microorganisms owing to the unique features of their …

A multifunctional thermophilic glycoside hydrolase from Caldicellulosiruptor owensensis with potential applications in production of biofuels and biochemicals

X Peng, H Su, S Mi, Y Han - Biotechnology for Biofuels, 2016 - Springer
Background Thermophilic enzymes have attracted much attention for their advantages of
high reaction velocity, exceptional thermostability, and decreased risk of contamination …