[HTML][HTML] Active human and murine tumor necrosis factor α cytokines produced from silkworm baculovirus expression system
T Ebihara, J Xu, Y Tonooka, T Nagasato, K Kakino… - Insects, 2021 - mdpi.com
Simple Summary Baculovirus expression vector system (BEVS) is widely employed to
produce eukaryotic recombinant proteins with desired post-translational modifications. The …
produce eukaryotic recombinant proteins with desired post-translational modifications. The …
Expression of recombinant human tumor necrosis factor-α in a baculovirus expression system
XW Zhang, T Sun, XY Zeng, X Liu, DX Gu - Bioresource technology, 1999 - Elsevier
A cDNA fragment coding human tumor necrosis factor-alpha (TNF-α) was inserted into the
vector pSXIVVI+ X3 with the control of Syn XIV promoter. The Sf9 cells (Spodoptera …
vector pSXIVVI+ X3 with the control of Syn XIV promoter. The Sf9 cells (Spodoptera …
Insect cell line dependent gene expression of recombinant human tumor necrosis factor-β
H Chai, M Al-Rubeai, KL Chua, SKW Oh… - Enzyme and microbial …, 1996 - Elsevier
The expression and secretion of recombinant human tumor necrosis factor-β (TNF-β
lymphotoxin) was examined in seven different insect host cell lines, namely, Spodoptera …
lymphotoxin) was examined in seven different insect host cell lines, namely, Spodoptera …
[HTML][HTML] Genetic engineering of baculovirus-insect cell system to improve protein production
M Hong, T Li, W Xue, S Zhang, L Cui… - … in Bioengineering and …, 2022 - frontiersin.org
The Baculovirus Expression Vector System (BEVS), a mature foreign protein expression
platform, has been available for decades, and has been effectively used in vaccine …
platform, has been available for decades, and has been effectively used in vaccine …
Human single-chain antibody expression in the hemolymph and fat body of silkworm larvae and pupae using BmNPV bacmids
M Ishikiriyama, T Nishina, T Kato, H Ueda… - Journal of bioscience and …, 2009 - Elsevier
Seven sets of recombinant expression vectors were constructed for the expression of the
human anti-bovine serum albumin (BSA) single-chain Fv fragment (scFv) 13CG2 fused to …
human anti-bovine serum albumin (BSA) single-chain Fv fragment (scFv) 13CG2 fused to …
Versatility of chitosan/BmNPV bacmid DNA nanocomplex as transfection reagent of recombinant protein expression in silkworm larvae
T Kato, S Arai, H Ichikawa, EY Park - Biotechnology letters, 2016 - Springer
Objective To examine the feasibility of chitosan as an alternative transfection reagent
candidate for protein expression in Bm5 cells and silkworm larvae using recombinant …
candidate for protein expression in Bm5 cells and silkworm larvae using recombinant …
[HTML][HTML] Characterization of chicken tumor necrosis factor-α, a long missed cytokine in birds
F Rohde, B Schusser, T Hron, H Farkašová… - Frontiers in …, 2018 - frontiersin.org
Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine playing critical roles in host defense
and acute and chronic inflammation. It has been described in fish, amphibians, and …
and acute and chronic inflammation. It has been described in fish, amphibians, and …
Establishment of the BacMam system using silkworm baculovirus
A Imai, T Tadokoro, S Kita, M Horiuchi… - Biochemical and …, 2016 - Elsevier
The BacMam system uses modified insect viruses (baculoviruses) as vehicles to efficiently
deliver genes for expression in mammalian cells. The technique can be widely applied to …
deliver genes for expression in mammalian cells. The technique can be widely applied to …
[HTML][HTML] Baculovirus-free insect cell expression system for high yield antibody and antigen production
J Korn, D Schäckermann, T Kirmann, F Bertoglio… - Scientific reports, 2020 - nature.com
Antibodies are essential tools for therapy and diagnostics. Yet, production remains
expensive as it is mostly done in mammalian expression systems. As most therapeutic IgG …
expensive as it is mostly done in mammalian expression systems. As most therapeutic IgG …
[HTML][HTML] Development of specific monoclonal antibodies for the detection of natural chicken tumor necrosis factor-alpha
Y Yang, Y Meng, L Chen, M Dong, H Zhang, J Wu… - Heliyon, 2022 - cell.com
Tumor necrosis factor alpha (TNF-α) is an important proinflammatory cytokine and the only
known cytokine that can directly kill tumor cells. Unlike mammalian counterparts, chicken …
known cytokine that can directly kill tumor cells. Unlike mammalian counterparts, chicken …