Genetic Code Expansion System for Tight Control of Gene Expression in Bombyx mori Cell Lines

W Lu, R Wang, P Wang, S Ma, Q Xia - Insects, 2021 - mdpi.com
Simple Summary Bombyx mori is a lepidopteran insect with economic value. Its genetic
background is clear, and genome sequence is relatively complete, but the function of many …

Construction of a piggyBac-based enhancer trap system for the analysis of gene function in silkworm Bombyx mori

K Uchino, H Sezutsu, M Imamura, I Kobayashi… - Insect Biochemistry and …, 2008 - Elsevier
Enhancer trapping and insertional mutagenesis are powerful tools for analyzing genetic
function. To construct an enhancer trap system in the silkworm Bombyx mori, we developed …

Construction and characterization of a synthetic Baculovirus-inducible 39K promoter

ZQ Dong, ZG Hu, HQ Li, YM Jiang, MY Cao… - Journal of Biological …, 2018 - Springer
Background Silkworm genetic engineering is widely used in gene function, silk engineering
and disease-resistant engineering in most of Asia. Some of the earliest promoter elements …

[HTML][HTML] Serial analysis of gene expression in the silkworm, Bombyx mori

J Huang, X Miao, W Jin, P Couble, K Mita, Y Zhang… - Genomics, 2005 - Elsevier
The silkworm Bombyx mori is one of the most economically important insects and serves as
a model for Lepidoptera insects. We used serial analysis of gene expression (SAGE) to …

Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori

N Karasaki, H Mon, M Takahashi, JM Lee, K Koga… - Biotechnology …, 2009 - Springer
Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices
for the control of transgenes expression in mammal and drosophila. However, the Tet-on …

Editing of the heavy chain gene of Bombyx mori using transcription activator like effector nucleases

Y Wang, M Nakagaki - Biochemical and Biophysical Research …, 2014 - Elsevier
The silk gland of Bombyx mori represents an established in vivo system for producing
recombinant proteins. However, low yields of recombinant proteins have limited the system's …

Effect of ATG initiation codon context motifs on the efficiency of translation of mRNA derived from exogenous genes in the transgenic silkworm, Bombyx mori

K Tatematsu, K Uchino, H Sezutsu, T Tamura - SpringerPlus, 2014 - Springer
The context sequence motif surrounding the ATG initiation codon influences mRNA
translation efficiency and affects protein production; however, the optimal sequence differs …

Identification of a Novel Strong and Ubiquitous Promoter/Enhancer in the Silkworm Bombyx mori

T Tsubota, K Uchino, TK Suzuki… - G3: Genes …, 2014 - academic.oup.com
Transgenic techniques offer a valuable tool for determining gene functions. Although various
promoters are available for use in gene overexpression, gene knockdown, and identification …

TIGHTLY CONTROLLED TETRACYCLINE‐INDUCIBLE TRANSCRIPTION SYSTEM FOR EXPLOSIVE GENE EXPRESSION IN CULTURED SILKWORM CELLS

T Tatsuke, JM Lee, T Kusakabe… - Archives of insect …, 2013 - Wiley Online Library
Tetracycline‐inducible gene expression (T et‐on) system is a particularly powerful tool for
transgenic research and has become one of the first choices for the control of transgene …

Performance of expression vector, pTD1, in insect cell-free translation system

T Suzuki, M Ito, T Ezure, S Kobayashi, M Shikata… - Journal of bioscience …, 2006 - Elsevier
We constructed a pTD1 vector for an insect cell-free translation system containing a 5′
untranslated region (UTR) of a polyhedrin gene as a translational enhancer sequence. Its …