[HTML][HTML] Time-resolved, deuterium-based fluxomics uncovers the hierarchy and dynamics of sugar processing by Pseudomonas putida
Pseudomonas putida, a microbial host widely adopted for metabolic engineering, processes
glucose through convergent peripheral pathways that ultimately yield 6-phosphogluconate …
glucose through convergent peripheral pathways that ultimately yield 6-phosphogluconate …
Multi-omics analysis unravels a segregated metabolic flux network that tunes co-utilization of sugar and aromatic carbons in Pseudomonas putida
Pseudomonas species thrive in different nutritional environments and can catabolize
divergent carbon substrates. These capabilities have important implications for the role of …
divergent carbon substrates. These capabilities have important implications for the role of …
[HTML][HTML] GC-MS-based 13C metabolic flux analysis resolves the parallel and cyclic glucose metabolism of Pseudomonas putida KT2440 and Pseudomonas …
M Kohlstedt, C Wittmann - Metabolic engineering, 2019 - Elsevier
The genus Pseudomonas comprises approximately 200 species with numerous isolates that
are common inhabitants of soil, water, and vegetation and has been of particular interest for …
are common inhabitants of soil, water, and vegetation and has been of particular interest for …
A metabolic widget adjusts the phosphoenolpyruvate-dependent fructose influx in Pseudomonas putida
Fructose uptake in the soil bacterium Pseudomonas putida occurs through a canonical
phosphoenolpyruvate (PEP)-dependent sugar transport system (PTSFru). The logic of the …
phosphoenolpyruvate (PEP)-dependent sugar transport system (PTSFru). The logic of the …
Redox stress reshapes carbon fluxes of Pseudomonas putida for cytosolic glucose oxidation and NADPH generation
The soil bacterium and metabolic engineering platform Pseudomonas putida tolerates high
levels of endogenous and exogenous oxidative stress, yet the ultimate reason of such …
levels of endogenous and exogenous oxidative stress, yet the ultimate reason of such …
A cyclic metabolic network in Pseudomonas protegens Pf-5 prioritizes the Entner-Doudoroff pathway and exhibits substrate hierarchy during carbohydrate co …
RA Wilkes, CM Mendonca… - Applied and environmental …, 2019 - Am Soc Microbiol
The genetic characterization of Pseudomonas protegens Pf-5 was recently completed.
However, the inferred metabolic network structure has not yet been evaluated …
However, the inferred metabolic network structure has not yet been evaluated …
Convergent Peripheral Pathways Catalyze Initial Glucose Catabolism in Pseudomonas putida: Genomic and Flux Analysis
T Del Castillo, JL Ramos… - Journal of …, 2007 - Am Soc Microbiol
In this study, we show that glucose catabolism in Pseudomonas putida occurs through the
simultaneous operation of three pathways that converge at the level of 6-phosphogluconate …
simultaneous operation of three pathways that converge at the level of 6-phosphogluconate …
Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol
V Beckers, I Poblete-Castro, J Tomasch… - Microbial cell …, 2016 - Springer
Background Given its high surplus and low cost, glycerol has emerged as interesting carbon
substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas …
substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas …
Analogous metabolic decoupling in Pseudomonas putida and Comamonas testosteroni implies energetic bypass to facilitate gluconeogenic growth
Gluconeogenic carbon metabolism is not well understood, especially within the context of
flux partitioning between energy generation and biomass production, despite the importance …
flux partitioning between energy generation and biomass production, despite the importance …
Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress
As a frequent inhabitant of sites polluted with toxic chemicals, the soil bacterium and plant-
root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous …
root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous …