[HTML][HTML] Time-resolved, deuterium-based fluxomics uncovers the hierarchy and dynamics of sugar processing by Pseudomonas putida

DC Volke, N Gurdo, R Milanesi, PI Nikel - Metabolic Engineering, 2023 - Elsevier
Pseudomonas putida, a microbial host widely adopted for metabolic engineering, processes
glucose through convergent peripheral pathways that ultimately yield 6-phosphogluconate …

Multi-omics analysis unravels a segregated metabolic flux network that tunes co-utilization of sugar and aromatic carbons in Pseudomonas putida

MA Kukurugya, CM Mendonca, M Solhtalab… - Journal of Biological …, 2019 - ASBMB
Pseudomonas species thrive in different nutritional environments and can catabolize
divergent carbon substrates. These capabilities have important implications for the role of …

[HTML][HTML] GC-MS-based 13C metabolic flux analysis resolves the parallel and cyclic glucose metabolism of Pseudomonas putida KT2440 and Pseudomonas …

M Kohlstedt, C Wittmann - Metabolic engineering, 2019 - Elsevier
The genus Pseudomonas comprises approximately 200 species with numerous isolates that
are common inhabitants of soil, water, and vegetation and has been of particular interest for …

A metabolic widget adjusts the phosphoenolpyruvate-dependent fructose influx in Pseudomonas putida

M Chavarría, Á Goñi-Moreno, V de Lorenzo, PI Nikel - Msystems, 2016 - Am Soc Microbiol
Fructose uptake in the soil bacterium Pseudomonas putida occurs through a canonical
phosphoenolpyruvate (PEP)-dependent sugar transport system (PTSFru). The logic of the …

Redox stress reshapes carbon fluxes of Pseudomonas putida for cytosolic glucose oxidation and NADPH generation

PI Nikel, T Fuhrer, M Chavarría, A Sánchez-Pascuala… - BioRxiv, 2020 - biorxiv.org
The soil bacterium and metabolic engineering platform Pseudomonas putida tolerates high
levels of endogenous and exogenous oxidative stress, yet the ultimate reason of such …

A cyclic metabolic network in Pseudomonas protegens Pf-5 prioritizes the Entner-Doudoroff pathway and exhibits substrate hierarchy during carbohydrate co …

RA Wilkes, CM Mendonca… - Applied and environmental …, 2019 - Am Soc Microbiol
The genetic characterization of Pseudomonas protegens Pf-5 was recently completed.
However, the inferred metabolic network structure has not yet been evaluated …

Convergent Peripheral Pathways Catalyze Initial Glucose Catabolism in Pseudomonas putida: Genomic and Flux Analysis

T Del Castillo, JL Ramos… - Journal of …, 2007 - Am Soc Microbiol
In this study, we show that glucose catabolism in Pseudomonas putida occurs through the
simultaneous operation of three pathways that converge at the level of 6-phosphogluconate …

Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol

V Beckers, I Poblete-Castro, J Tomasch… - Microbial cell …, 2016 - Springer
Background Given its high surplus and low cost, glycerol has emerged as interesting carbon
substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas …

Analogous metabolic decoupling in Pseudomonas putida and Comamonas testosteroni implies energetic bypass to facilitate gluconeogenic growth

RA Wilkes, J Waldbauer, L Aristilde - MBio, 2021 - Am Soc Microbiol
Gluconeogenic carbon metabolism is not well understood, especially within the context of
flux partitioning between energy generation and biomass production, despite the importance …

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

PI Nikel, T Fuhrer, M Chavarría… - The ISME …, 2021 - academic.oup.com
As a frequent inhabitant of sites polluted with toxic chemicals, the soil bacterium and plant-
root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous …