… Moreover, the strategy developed in this study combining approaches of ALE, genome
resequencing, RNA-Seq, and classical genetics study for mutant evolution and characterization …

… We chose fusions that could tolerate 3.0 g/L furfural for further analysis of fermentation
and whole-genome sequencing. Ten strains were involved, including the first round of …

… xylose utilization including those using Zymomonas mobilis as the host. … mobilis strains
that can utilize xylose efficiently at high concentrations, and NGS-based genome resequencing …

… efficient genome engineering of an important industrial microorganism, Zymomonas mobilis…
This toolkit included a series of genome engineering plasmids, each carrying an artificial self…

… To validate mutations identified in the genome sequencing, DNA regions containing
mutated genes were amplified by PCR and sequenced using the Sanger method at Macrogen …

… pathway by integrating necessary genes in genomes or by their heterologous expression …
Dunn and Rao have followed a similar approach, where high-throughput sequencing was …

… mutants to gene disruption events via barcode sequencing, an … barcode sequencing
techniques such as Bar-seq [27], which enables the determination of a mutant’s, and thereby a gene’…

Metabolic engineering of the biofuel-producing Zymomonas mobilis is … iterative genome
modification. Here, we have developed an efficient method for generating markerless genomic …

… Zymomonas mobilis is becoming a popular bacterial host for biofuel research due to its fast
… Sequencing of Zymomonas genomes The draft genomes of Zymomonas mobilis strains …

… sequencing, we assembled the genome of ZM401 to explore genetic basis for the self-flocculation
of the bacterial cells through comparative genomic analysis with the genome … genome …