[HTML][HTML] A novel culture medium with reduced nutrient concentrations supports the development and viability of mouse embryos

AF Ermisch, JR Herrick, R Pasquariello, MKC Dyer… - Scientific reports, 2020 - nature.com
… than what is supplied in traditional culture media. Our objective was to determine the
impact of reduced nutrient concentrations in culture medium on mouse embryo development, …

[HTML][HTML] Selected amino acids promote mouse pre-implantation embryo development in a growth factor-like manner

MB Morris, S Ozsoy, M Zada, M Zada… - Frontiers in …, 2020 - frontiersin.org
… , mouse embryos provide autocrine/paracrine support for … We note that mTOR –/– mouse
embryos exhibit reduced … rational design of improved culture media for embryos depends on …

Embryo development and culture techniques

DK Gardner - Animal Breeding, 2021 - taylorfrancis.com
embryo culture media, capable of supporting high levels of … The concentration of all individual
components of the fluids of the … , such media with serum support very low levels of embryo

Mouse embryo assay for human in vitro fertilization quality control: a fresh look

N Esfandiari, A Gubista - Journal of Assisted Reproduction and Genetics, 2020 - Springer
culture mouse embryos in two different culture media received from AAB to determine which
media supports the in vitro growth of embryoslimited extent such that unfavorable media is …

[HTML][HTML] Development of serum-free and grain-derived-nutrient-free medium using microalga-derived nutrients and mammalian cell-secreted growth factors for …

K Yamanaka, Y Haraguchi, H Takahashi… - Scientific Reports, 2023 - nature.com
… You are using a browser version with limited support for CSS. … Here, we developed a novel
medium containing nutrients … wherein the nutrients in the culture medium are provided by a …

[HTML][HTML] Redox Regulation and Oxidative Stress in Mammalian Oocytes and Embryos Developed In Vivo and In Vitro

MLM Hardy, ML Day, MB Morris - International Journal of Environmental …, 2021 - mdpi.com
support for the developing embryo and, consistent with this, co-… to mouse embryo culture
medium reduces ROS in these … reduction in the concentration of intrafollicular concentration of 8-…

[HTML][HTML] Challenges and considerations during in vitro production of porcine embryos

PR Chen, BK Redel, KC Kerns, LD Spate, RS Prather - Cells, 2021 - mdpi.com
… -stage embryos is decreased compared to those that developed … shown to support development
of porcine embryos to the … Furthermore, early mouse and hamster embryo culture media

mTORC1/2 signaling is downregulated by amino acid-free culture of mouse preimplantation embryos and is only partially restored by amino acid readdition

RC Zamfirescu, ML Day… - American Journal of …, 2021 - journals.physiology.org
… the culture medium, even for a few hours, decreased mTORC1 … simple media lacking amino
acids (AAs) supported embryo … to the mod-HTF medium to a final concentration of 0.4 mM. All …

Antioxidants increase blastocyst cryosurvival and viability post-vitrification

TT Truong, DK Gardner - Human reproduction, 2020 - academic.oup.com
embryo culture media has significant beneficial effects on mouse embryo and fetal development
and A3 ×200 antioxidant concentrations resulted in significantly lower total cell numbers (…

[HTML][HTML] Murine blastocysts release mature MicroRNAs into culture media that reflect developmental status

DC Hawke, DB Ahmed, AJ Watson, DH Betts - Frontiers in Genetics, 2021 - frontiersin.org
support the use of extracellular miRNA to assess embryonic health and enable … of low
embryo-to-culture media volume ratios, resulting in increased dilution and reduced concentration