N-Linked glycosylation prevents deamidation of glycopeptide and glycoprotein
… Our results presented that deamidation is prevented by naturally occurring N-linked … that
N-linked glycosylation prevented deamidation both in glycoprotein and in glycopeptide. As …
N-linked glycosylation prevented deamidation both in glycoprotein and in glycopeptide. As …
[HTML][HTML] Reducing interferences in glycosylation site mapping
L Birx, A Harvey, M Popov… - Journal of Biomolecular …, 2022 - ncbi.nlm.nih.gov
… N-linked glycosylation on a protein involves the identification of deamidated sites after releasing
the glycans … is highlighted again in the chromatogram of the representative glycopeptide …
the glycans … is highlighted again in the chromatogram of the representative glycopeptide …
Deglycosylation by the Acidic Glycosidase PNGase H+ Enables Analysis of N-Linked Glycoproteins by Hydrogen/Deuterium Exchange Mass Spectrometry
G Comamala, JB Madsen, J Voglmeir… - Journal of the …, 2020 - ACS Publications
… of its activity below pH 6, which prevents its use at the quench … , the identification of
glycopeptides would require additional … By separating the amidated and deamidated species, we …
glycopeptides would require additional … By separating the amidated and deamidated species, we …
Removal of variable domain N-linked glycosylation as a means to improve the homogeneity of HIV-1 broadly neutralizing antibodies
… as products for treating or preventing infection by HIV-1. … the heavy chain (Fc) glycopeptides
are observed in the PGT121… of endogenous or the deamidation possibly induced by the …
are observed in the PGT121… of endogenous or the deamidation possibly induced by the …
N-Glycosylation of monoclonal antibody therapeutics: A comprehensive review on significance and characterization
… The N-linked glycans share a typical core pentasaccharide … of intact glycoprotein, middle-up,
glycopeptides and released … to be useful in identifying deamidated variants of several mAbs…
glycopeptides and released … to be useful in identifying deamidated variants of several mAbs…
[HTML][HTML] Depolarization-dependent induction of site-specific changes in sialylation on N-linked glycoproteins in rat nerve terminals
I Boll, P Jensen, V Schwämmle, MR Larsen - Molecular & Cellular …, 2020 - ASBMB
… Here we show that sialylation of N-linked glycosylation is a … of formerly sialylated N-linked
glycopeptides. We suggest that … and function of membrane glycoproteins. We characterized …
glycopeptides. We suggest that … and function of membrane glycoproteins. We characterized …
DeGlyPHER: Highly sensitive site-specific analysis of N-linked glycans on proteins
S Baboo, JK Diedrich, S Martínez-Bartolomé… - Methods in …, 2023 - Elsevier
… These limitations have prevented glycoproteomics from being adapted to high-throughput …
intact glycoprotein (before PK digestion) and then to deglycosylate glycopeptides generated …
intact glycoprotein (before PK digestion) and then to deglycosylate glycopeptides generated …
[HTML][HTML] … Glycosylation of Adeno-Associated Virus Serotype 9 Capsid Proteins Generated from HEK293 Cells through Glycopeptide Mapping and Released Glycan …
… remove almost all N-linked glycans from glycoproteins, there … of non-enveloped hepatitis
E virus prevented the formation of … with no deamidation (VP3 peak a), with deamidation (VP3 …
E virus prevented the formation of … with no deamidation (VP3 peak a), with deamidation (VP3 …
Site-Specific N-Linked Glycosylation Analysis of Human Carcinoembryonic Antigen by Sheathless Capillary Electrophoresis–Tandem Mass Spectrometry
L Pont, V Kuzyk, F Benavente… - Journal of proteome …, 2021 - ACS Publications
… as cancer biomarkers is not surprising and keeps driving the … N) can undergo spontaneous
deamidation both in vivo and in … -glycosylation sites were characterized using N-glycopeptide …
deamidation both in vivo and in … -glycosylation sites were characterized using N-glycopeptide …
Systems-wide analysis of glycoprotein conformational changes by limited deglycosylation assay
SN Mule, L Rosa-Fernandes, JVP Coutinho… - Journal of …, 2021 - Elsevier
… that prevent unfolded, misfolded and aggregated glycoproteins to … In this study, to ensure
high confidence of the deamidated … robustness of the N-linked glycopeptide enrichment and site …
high confidence of the deamidated … robustness of the N-linked glycopeptide enrichment and site …