… We report here a novel in vitro experimental system, the metabolism-dependent … metabolism
in toxicity. MDCA employs permeabilized cofactor-supplemented cryopreserved human …

… We report here a novel in vitro experimental system, the metabolism-dependent … metabolism
in toxicity. MDCA employs permeabilized cofactor-supplemented cryopreserved human …

… a novel in vitro hepatic metabolic system, the permeabilized … , MMHH), as an exogenous
metabolic activating system for … from cryopreserved human hepatocytes isolated from human …

… both endogenous and exogenous substances. Consequently… established with primary
human hepatocytes obtained from … , we developed a novel 2D HH culture system with stepwise …

… quantities of viable hepatocytes of high quality, with excellent metabolic functionality. There
is … In cryopreserved human hepatocytes, there was a decrease in oxygen consumption rate …

… to expand or cryopreserve gene-edited hepatocytes for … of our strategy, we explore a
novel cell-based gene editing … into the primary mouse and human hepatocytes as a highly …

… hepatocyte phenotype with altered transcription factor expression, low metabolic capacities
in comparison with primary hepatocytes and … Finally, we have cryopreserved cell pellets to …

… and lysed hepatocytes in the presence of exogenous NADPH. … Cryopreserved HH were
rapidly thawed in a 37C water bath … intact and permeabilized rat hepatocytes demonstrates that …

… Primary human hepatocytes (PHHs) cannot survive for more … between rodent and human
metabolism enzymes, results … matrix, fresh or cryopreserved hepatocytes were first incubated …

… (1) the metabolism of endogenous substrates and exogenous … performed directly by seeding
frozen/cryopreserved iPSCs. An … Aspirate the permeabilization buffer and add appropriate …