… of the homologous arms that it generates for recombination-… gene targeting is mainly for
targeting protein-coding genes as … These fragments were further integrated into an intermediate …

… Lower Left: Targeted amplification, or PCR-based techniques… favors the amplification of
high-abundance alleles in a mixed … Alternatively cleaved DNA can be integrated into long-read …

… a homology-directed insertion method to knockout genes in the … study are listed in Table 1
(Integrated DNA Technologies) … using a PCR-based strategy without any need for sequencing. …

… , since these short homology flanking regions of 40 bps can … PCR-based quantification
(RT-qPCR), which allows a more … independent experiments targeting two different genes located …

… DNA break at the target intronic region, usually cleaving at 3 … genome targeting with a dual
surrogate reporter-integrated … in the mutant allele then are repaired through homologous …

… We compared PCR-based and nCATs targeted sequencing … to produce BAM files that
can be visualised in Integrative … black areas highlight the homology between the two alleles …

… regions homologous to the desired genomic loci and the cassette is integrated by homologous
… (in parenthesis are the genes that the gRNAs of each plasmid are targeting): 3×_gRNA (…

… Sequences that showed homology to any of the reference … loci) are located in or within 2
kb of genes [16]. Most of the 35 … showed high amplification efficiency, and were not integrated …

… shorter homology regions were also sufficient for gene targeting. We chose the H. uvarum
LEU2 and LYS2 genes as targets to investigate PCR-based gene targeting … had integrated the …

… target a range of homologous sequences, making the same set of SSPs suitable for GW with
a series of related species or genes… All PCR-based methods for GW are potentially sensitive …