… Here, 20 using targeting the mouse trypsinogen genes as an … Traditionally, homologous
recombination in embryonic stem … 231 In this study, we established a PCR-based method to …

… Gene targeting by homologous recombination has been … for the integrated expression of
heterologous genes in this … recombination requires long regions of homology to target sites and …

… The entire regions of most target genes could be amplified … by variants that increase their
homologies, which is suggestive of a … The LR PCR products used in this study were generated …

… pads with methods such as homology-directed repair (HDR) … -targeting LSRs from our
pipeline, Pc01 and Enc9, integrated … strategy, we calculated the distance between target genes …

… To further increase the speed of generating transgenic lines, … with homology arms matching
the targeted genomic region … terminator were integrated into the target genome site as part of …

… to identify primers for PCR based on several search criteria … or designing donor DNA for
homology-directed repair (HDR), … may derive from any randomly integrated flox sequence. In …

… types of alleles, we first consider general strategies and then … clones that have integrated
the gene-targeting vector is usually … a probe within the region of targeting vector homology (eg, …

… Since nLA and nRA sequences are homologous to the … clones included the randomly
integrated clones in conditions such … appropriate for generating models of polymorphic genes like …

… Summarizing, PCR-based isolation of large deletion alleles is feasible with the used … genes
in each linkage group share high sequence homology, and sgRNAs were designed to target …

… template contains homology arms flanking the region to be … of the allele must include a
PCR-based genotyping strategy to … We typically order oligonucleotides from Integrated DNA …