… extract methods, with a specific focus on prokaryotic systems. Firstly, we describe the diversity
of Escherichia … We then trace the history of cell-extract methodology over the past 20 years, …

… One of the main factors affecting metabolic burden is promoter strength. lac-derived
promoters (P T7 lac , P trc , and P tac ) are all based on the negative regulation by LacI, and the …

… In this study, we optimised anaerobic production of ethyl acetate in Escherichia coli. We
compared and evaluated ethyl acetate production by three AATs from different yeasts after …

… The resulting ec_iML1515 model showed that the protein demand and protein synthesis
rate were the key factors affecting lysine production. By optimizing the expression of the 20 top-…

… and analysis of transcription factor regulons that were reconstructed by the comparative
genomic approach. The database contains a taxonomic set of transcription factors from over 25 …

… planktonic and biofilm cells of Escherichia coli JM109(DE3) transformed with a plasmid
containing a T7 promoter. It was shown that induction negatively affected the growth and viability …

… Here we employ Escherichia coli membranes containing KcsA as a model protein to investigate
the influence of different environmental conditions on SMA solubilization efficiency. We …

… Orange 2 dye degradation using Escherichia coli. This study … both in one time factor
optimization and optimization using central … As a consequence, this optimization technique does …

… codon optimization method … optimization can be converted to sequence annotation of
corresponding amino acids with codon boxes. The codon optimization models for Escherichia Coli …

… In this study, Escherichia coli was metabolically engineered to efficiently produce LNT II
using glycerol as a carbon source and lactose as a substrate. The UDP-N-acetylglucosamine (…