… and versatile for single-molecule sensing applications … a practical guide to their use,
focusing on the single-molecule … to perform successful single-molecule experiments, including …

… At the single-molecule level FRET is sensitive to heterogeneous subpopulations, can …
inter-laboratory benchmarking study 5 , absolute FRET efficiencies can be used to infer precise …

… Often, conformational changes and molecular interactions are difficult or impossible to
synchronize, or too rare or transient to detect using ensemble FRET. Single-molecule FRET (…

… Single-molecule FRET measurements were combined with modeling to allow for the
determination of rate constants for the initial formation of Type A oligomers and subsequent …

… FRET efficiency E values from the photon counts detected in the donor (D) and acceptor (A)
detection channels during a single-molecule … and exclusion of single-molecule events from …

… This in turn makes single-molecule FRET an interesting tool for dynamic structural …
single-molecule FRET spectroscopy and the added information it gives compared to ensemble FRET …

… heterogeneities in single-molecule FRET. (a) Simulated single-molecule interconverting
between … Identifying conformational dynamics and heterogeneities in single-molecule FRET. (a) …

… to the methodology associated with single-molecule FRET, with a … practical considerations
related to this integrative approach. … combinations of single-molecule FRET and biomolecular …

… To study this process, we developed a single molecule FRET assay to monitor the conformation
of the sgRNA and the binding of Cas9 to sgRNA. We labeled the sgRNA with a donor …

… Single-molecule FRET (smFRET) is a powerful imaging platform capable of revealing
dynamic changes in the conformation and proximity of biological molecules. The expansion of …