[HTML][HTML] Development of Genetic Tools in Glucoamylase-Hyperproducing Industrial Aspergillus niger Strains
D Liu, Q Liu, W Guo, Y Liu, M Wu, Y Zhang, J Li, W Sun… - Biology, 2022 - mdpi.com
… The filamentous fungus Aspergillus niger is widely exploited … and Aspergillus nidulans, genetic
transformation methods have … We provided high-efficiency genetic tools via the systematic …
transformation methods have … We provided high-efficiency genetic tools via the systematic …
[HTML][HTML] A Mad7 system for genetic engineering of filamentous fungi
KG Vanegas, JKH Rendsvig, ZD Jarczynska… - Journal of Fungi, 2022 - mdpi.com
… engineering of four different Aspergillus species: Aspergillus nidulans, A. niger, A. oryzae …
technology in fungi promises to reduce this barrier, as efficient gene-editing methods based on …
technology in fungi promises to reduce this barrier, as efficient gene-editing methods based on …
Strain breeding and gene complementation strategies for filamentous fungi
PA Wang, JJ Zhong - Process Biochemistry, 2023 - Elsevier
… methods, gene complementation strategies and biotechnological application of gene
complementation for filamentous fungi. In addition, we discuss the challenges to current gene …
complementation for filamentous fungi. In addition, we discuss the challenges to current gene …
… free gene replacement in citric acid-producing Aspergillus tubingensis (A. niger) WU-2223L by the CRISPR/Cas9 system-based genome editing technique using DNA …
I Yoshioka, K Kirimura - Journal of bioscience and bioengineering, 2021 - Elsevier
… enable rapid and efficient gene replacement in citric acid-producing fungi for genetic breeding.
… via fermentation using filamentous fungi belonging to Aspergillus section Nigri, such as …
… via fermentation using filamentous fungi belonging to Aspergillus section Nigri, such as …
Genetic Manipulation and Transformation Methods for Aspergillus spp.
… tumefaciens is used as a vector for gene transfer in various filamentous fungi. AMT was first
… available gene editing methods and transformation efficiency are low, but filamentous fungi …
… available gene editing methods and transformation efficiency are low, but filamentous fungi …
Development of a new Agrobacterium-mediated transformation system based on a dual auxotrophic approach in the filamentous fungus Aspergillus oryzae
… We further established a new ATMT system based on the dual auxotrophic strains with the
use of hisB and pyrG as the selectable markers for efficient genetic manipulations in A. oryzae…
use of hisB and pyrG as the selectable markers for efficient genetic manipulations in A. oryzae…
[HTML][HTML] Development of a marker-free mutagenesis system using CRISPR-Cas9 in the pathogenic mould Aspergillus fumigatus
N Van Rhijn, T Furukawa, C Zhao, BL McCann… - Fungal Genetics and …, 2020 - Elsevier
… for our ability to perform genetic manipulation in filamentous fungi, most notably because
they allow … The first systems to be employed in filamentous fungi used in vivo expressed Cas9 …
they allow … The first systems to be employed in filamentous fungi used in vivo expressed Cas9 …
CRISPR/Cas9 technology enables the development of the filamentous ascomycete fungus Penicillium subrubescens as a new industrial enzyme producer
S Salazar-Cerezo, RS Kun, RP De Vries… - Enzyme and microbial …, 2020 - Elsevier
… in filamentous fungi for gene editing because of its high … an efficient genetic transformation
method for the filamentous … niger, Aspergillus nidulans, Aspergillus acuelatus, T. reesei, …
method for the filamentous … niger, Aspergillus nidulans, Aspergillus acuelatus, T. reesei, …
Development of a cost-effective medium for submerged production of fungal aryl alcohol oxidase using a genetically modified Aspergillus nidulans strain
… This study provides a potential and promising solution for cost-effective production of AAO
and provides an inexpensive medium for fungal enzyme production using filamentous fungi. …
and provides an inexpensive medium for fungal enzyme production using filamentous fungi. …
Application of CRISPR in filamentous fungi and macrofungi: from component function to development potentiality
JY Shen, Q Zhao, QL He - ACS Synthetic Biology, 2023 - ACS Publications
… This study provides an effective one-step traceless editing strategy for gene … genes in
filamentous fungi. In addition to cas9 nucleases, studies on gene editing in filamentous fungi using …
filamentous fungi. In addition to cas9 nucleases, studies on gene editing in filamentous fungi using …