[HTML][HTML] Development of Genetic Tools in Glucoamylase-Hyperproducing Industrial Aspergillus niger Strains

D Liu, Q Liu, W Guo, Y Liu, M Wu, Y Zhang, J Li, W Sun… - Biology, 2022 - mdpi.com
… The filamentous fungus Aspergillus niger is widely exploited … and Aspergillus nidulans, genetic
transformation methods have … We provided high-efficiency genetic tools via the systematic …

[HTML][HTML] A Mad7 system for genetic engineering of filamentous fungi

KG Vanegas, JKH Rendsvig, ZD Jarczynska… - Journal of Fungi, 2022 - mdpi.com
… engineering of four different Aspergillus species: Aspergillus nidulans, A. niger, A. oryzae …
technology in fungi promises to reduce this barrier, as efficient gene-editing methods based on …

Strain breeding and gene complementation strategies for filamentous fungi

PA Wang, JJ Zhong - Process Biochemistry, 2023 - Elsevier
methods, gene complementation strategies and biotechnological application of gene
complementation for filamentous fungi. In addition, we discuss the challenges to current gene

… free gene replacement in citric acid-producing Aspergillus tubingensis (A. niger) WU-2223L by the CRISPR/Cas9 system-based genome editing technique using DNA …

I Yoshioka, K Kirimura - Journal of bioscience and bioengineering, 2021 - Elsevier
… enable rapid and efficient gene replacement in citric acid-producing fungi for genetic breeding.
… via fermentation using filamentous fungi belonging to Aspergillus section Nigri, such as …

Genetic Manipulation and Transformation Methods for Aspergillus spp.

YE Son, HS Park - Mycobiology, 2021 - Taylor & Francis
… tumefaciens is used as a vector for gene transfer in various filamentous fungi. AMT was first
… available gene editing methods and transformation efficiency are low, but filamentous fungi

Development of a new Agrobacterium-mediated transformation system based on a dual auxotrophic approach in the filamentous fungus Aspergillus oryzae

HD Thai, BPT Nguyen, VM Nguyen, QH Nguyen… - World Journal of …, 2021 - Springer
… We further established a new ATMT system based on the dual auxotrophic strains with the
use of hisB and pyrG as the selectable markers for efficient genetic manipulations in A. oryzae…

[HTML][HTML] Development of a marker-free mutagenesis system using CRISPR-Cas9 in the pathogenic mould Aspergillus fumigatus

N Van Rhijn, T Furukawa, C Zhao, BL McCann… - Fungal Genetics and …, 2020 - Elsevier
… for our ability to perform genetic manipulation in filamentous fungi, most notably because
they allow … The first systems to be employed in filamentous fungi used in vivo expressed Cas9 …

CRISPR/Cas9 technology enables the development of the filamentous ascomycete fungus Penicillium subrubescens as a new industrial enzyme producer

S Salazar-Cerezo, RS Kun, RP De Vries… - Enzyme and microbial …, 2020 - Elsevier
… in filamentous fungi for gene editing because of its high … an efficient genetic transformation
method for the filamentous … niger, Aspergillus nidulans, Aspergillus acuelatus, T. reesei, …

Development of a cost-effective medium for submerged production of fungal aryl alcohol oxidase using a genetically modified Aspergillus nidulans strain

E Liu, M Li, A Abdella, MR Wilkins - Bioresource Technology, 2020 - Elsevier
… This study provides a potential and promising solution for cost-effective production of AAO
and provides an inexpensive medium for fungal enzyme production using filamentous fungi. …

Application of CRISPR in filamentous fungi and macrofungi: from component function to development potentiality

JY Shen, Q Zhao, QL He - ACS Synthetic Biology, 2023 - ACS Publications
… This study provides an effective one-step traceless editing strategy for genegenes in
filamentous fungi. In addition to cas9 nucleases, studies on gene editing in filamentous fungi using …