Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans Using Episomal Vectors

I Roux, YH Chooi - Engineering Natural Product Biosynthesis: Methods …, 2022 - Springer
Filamentous fungal hosts are often preferred for the expression of BGCs from filamentous
fungi… secretion systems, and overall better genetic compatibility than bacterial or yeast hosts [3, …

A versatile in vivo DNA assembly toolbox for fungal strain engineering

ZD Jarczynska, K Garcia Vanegas… - ACS Synthetic …, 2022 - ACS Publications
… end-joining deficient strains of Aspergillus nidulans, efficient fusions can be … efficient gene
targeting in filamentous fungi, even in NHEJ-deficient strains, requires longer stretches of DNA

NHEJ and HDR can occur simultaneously during gene integration into the genome of Aspergillus niger

S Fritsche, A Reinfurt, F Fronek, MG Steiger - Fungal Biology and …, 2024 - Springer
… The filamentous fungus Aspergillus niger is an important cell factory in the … gene and argB
gene of Aspergillus nidulans, and the pyr4 … Both strategies are recognized as efficient genetic

The Function of the XlnR Transcription Factor in the Filamentous Fungus Penicillium verruculosum

VY Kislitsin, AM Chulkin, IN Zorov, IG Sinelnikov… - Applied Biochemistry …, 2023 - Springer
… the Aspergillus aculeatus filamentous fungus genome … technique is widely used to improve
the technological characteristics of industrially important filamentous fungi of the Aspergillus [6…

CRISPR/Cas9-Based Genome Editing and Its Application in Aspergillus Species

FJ Jin, BT Wang, ZD Wang, L Jin, P Han - Journal of Fungi, 2022 - mdpi.com
Aspergillus, a genus of filamentous fungi, is extensively distributed in nature and plays crucial
… Within the genus, Aspergillus nidulans has received widespread recognition as a model …

FungalBraid 2.0: expanding the synthetic biology toolbox for the biotechnological exploitation of filamentous fungi

E Moreno-Giménez, M Gandía, Z Sáez… - … in bioengineering and …, 2023 - frontiersin.org
… -to-use genetic elements, and universal syntax and rules for contributory use by the fungal
… have been developed and applied to filamentous fungi, which highlights the relevance of …

A Novel Site-Specific Integration System for Genetic Modification of Aspergillus flavus

F Tao, K Zhao, Q Zhao, F Xiang… - G3: Genes, Genomes …, 2020 - academic.oup.com
… , ectopic recombination for DNA integration into the genomes of filamentous fungi. Indeed,
work has focused on improving the frequency of gene targeting by homologous recombination…

Expression of microbial lipase in filamentous fungus Aspergillus niger: a review

H Nie, Y Zhang, M Li, W Wang, Z Wang, J Zheng - 3 Biotech, 2024 - Springer
… This genetic engineering technique has been used to modify the genetic makeup of
filamentous fungi, such as Fusarium fujikuroi, Myceliophthora, A. niger, and others (Table 4). …

Visualized Multigene Editing System for Aspergillus niger

C Li, J Zhou, S Rao, G Du, S Liu - ACS Synthetic Biology, 2021 - ACS Publications
efficiency of multigene editing in filamentous fungi, researchers have developed the cassette
to transcribe and process … of PgpdA (the promoter of Aspergillus nidulans glyceraldehyde-3-…

[PDF][PDF] CRISPR-mediated, vector-based, marker-free genome editing tool for filamentous fungi

L Mózsik, RAL Bovenberg, Y Nygård… - … in Filamentous Fungi, 2022 - research.rug.nl
… Here we report on a vector-based CRISPR/Cas9 system for filamentous fungi, which
enables rapid sgRNA library assembly and efficient, precise genome editing. The vector system …