Development of a cost-effective medium for submerged production of fungal aryl alcohol oxidase using a genetically modified Aspergillus nidulans strain

E Liu, M Li, A Abdella, MR Wilkins - Bioresource Technology, 2020 - Elsevier
… This study provides a potential and promising solution for cost-effective production of AAO
and provides an inexpensive medium for fungal enzyme production using filamentous fungi. …

CRISPR/Cas9-Based Genome Editing in the Filamentous Fungus Glarea lozoyensis and Its Application in Manipulating gloF

TY Wei, YJ Wu, QP Xie, JW Tang, ZT Yu… - ACS Synthetic …, 2020 - ACS Publications
… Glarea lozoyensis, an important industrial filamentous fungusefficiency gene mutagenesis
in the target gene via nonhomologous end joining (NHEJ)-mediated events during DSB repair

CRISPR-Cas9 system for functional genomics of filamentous fungi: applications and challenges

A Kumar, S Chadha, D Rath - Fungi bio-prospects in sustainable …, 2021 - Elsevier
methods (Fang and Wang, 2016). This chapter describes the CRISPR-Cas9 genome editing
tool and its relevance to filamentous fungi. … in Aspergillus nidulans by targeting the yA gene. …

Engineering interventions in industrial filamentous fungal cell factories for biomass valorization

A Madhavan, KB Arun, R Sindhu, AA Jose… - Bioresource …, 2022 - Elsevier
… current trends in engineering filamentous fungi for enzymes, … that filamentous fungi produce,
engineering of filamentous fungiAspergillus nidulans, Aspergillus oryzae, Aspergillus niger, …

Protein expression and secretion by filamentous fungi

AA Sakekar, SR Gaikwad, NS Punekar - Journal of Biosciences, 2021 - Springer
… In fact A niger and Aspergillus oryzae are the … filamentous fungi (AMFEP 2015). Much
research on protein expression/secretion involves these two Aspergilli with Aspergillus nidulans (…

In the fungus where it happens: history and future propelling Aspergillus nidulans as the archetype of natural products research

LK Caesar, NL Kelleher, NP Keller - Fungal Genetics and Biology, 2020 - Elsevier
… Complementation and subsequent sequencing of one of these mutants revealed the ORF
laeA, unique to filamentous fungi. Deletion of laeA blocked expression of numerous known …

CRISPR/Cas9-mediated genome editing directed by a 5S rRNA–tRNAGly hybrid promoter in the thermophilic filamentous fungus Humicola insolens

C Fan, W Zhang, X Su, W Ji, H Luo, Y Zhang… - Biotechnology for …, 2021 - Springer
CRISPR/Cas9-mediated genome editing directed by a 5S rRNA–tRNAGly hybrid promoter
in the thermophilic filamentous fungus Humicola insolens | Biotechnology for Biofuels and …

Current genetic strategies to investigate gene functions in Trichoderma reesei

C Ma, J Liu, J Tang, Y Sun, X Jiang, T Zhang… - Microbial Cell …, 2023 - Springer
… been widely applied for genome editing in filamentous fungi [101, 102]. The efficient gene
editing mediated by SpCas9 at DNA sequences requires an NGG protospacer adjacent motif (…

A versatile in vivo dna assembly toolbox for fungal strain engineering

ZD Jarczynska, K Garcia Vanegas… - ACS Synthetic …, 2022 - ACS Publications
… end-joining deficient strains of Aspergillus nidulans, efficient fusions can be … efficient gene
targeting in filamentous fungi, even in NHEJ-deficient strains, requires longer stretches of DNA

Polystyrene upcycling into fungal natural products and a biocontrol agent

C Rabot, Y Chen, SY Lin, B Miller… - Journal of the …, 2023 - ACS Publications
… Herein, we apply a similar reaction to rapidly convert PS to benzoic acid in high yield.
Engineered strains of the filamentous fungus Aspergillus nidulans then biosynthetically upgrade …