… coding for amino acids coordinating the two divalent metals in human proteins have the
capacity to confer a significantly lower exon inclusion for tight Zn 2+ than for weak Ca 2+ . …

… metal ions, while the divalent metal ions were … ionic concentrations of 100 mM for magnesium
ions and 150 mM for potassium ions, in line with the crystallization conditions of the intron (…

… were performed for Mg 2+ and Ca 2+ bound active site of group II intron in pre-hydrolytic, …
Representative MD structures of group II intron were selected and divalent metal ions M1, M2 …

… states by employing two levels of theory (M06–2X/6-311++G** and B3LYP/6-31+G*). ΔE
approximately captures the divalent metal-ion preference in the lariat group II intron. The …

… We survey the history of group II intron studies paralleling the trends in RNA methodology
… analysis that these two reactions compete with each other.[37] Two divalent metal ions (M1 …

… to that of many other divalent metal ion-dependent DNA/RNA … the two divalent ions (M1
and M2) chelated by acidic groups … two positively charged residues (K1 and K2) located in the …

… promoting the forward splicing reaction. Comparison with group II introns suggests its enzymatic
… believed and not reliant solely on divalent metal ions. The heterogeneous nature of the …

… of the involvement of catalytic divalent metal ions in nonenzymatic RNA primer extension. …
of the catalytic metal ion in the primer extension reaction center, we first synthesized 2-…

… domains in canonical group II introns (Figure 2). D5 is the catalytic center of group II ribozymes,
positioning two mechanistically critical divalent metal ions via interactions between the …

… Mg 2+ ions at the RNase H1 reaction center. We found that such … Here, K U is replaced by
the third divalent metal ion M C (… group II intron ribozymes (two K + , K 1 –K 2 ; and two Mg 2+ …