[HTML][HTML] Optimization of a method to detect autoantigen-specific T-cell responses in type 1 diabetes

Y Musthaffa, HJ Nel, N Ramnoruth, S Patel… - Frontiers in …, 2020 - frontiersin.org
sensitivity of a published CFSE-based T-cell proliferation assay that demonstrated proinsulin
33-63 –specific CD4 + T cells … CFSE-based T-cell proliferation assay targeting rare antigen-…

[HTML][HTML] Guidelines for analysis of low-frequency antigen-specific T cell results: Dye-based proliferation assay vs 3H-thymidine incorporation

D Di Blasi, I Claessen, AW Turksma, J van Beek… - … immunological methods, 2020 - Elsevier
… data interpretation for results of proliferation assays. Here we … -off for detecting the response
of low frequency Ag-specific T cells, … assay is sensitive enough to detect an Ag-specific T cell …

[HTML][HTML] High-throughput detection of autoantigen-specific b cells among distinct functional subsets in autoimmune donors

BA Joosse, JH Jackson, A Cisneros 3rd… - Frontiers in …, 2021 - frontiersin.org
T cells to drive their activation, proliferation, and effector cell differentiation and/or by differentiating
into autoantibody-secreting cells… repertoire that are as rare as 1 in 20 million cells (29)…

[HTML][HTML] Precision targeting of autoantigen-specific B cells in muscle-specific tyrosine kinase myasthenia gravis with chimeric autoantibody receptor T cells

S Oh, X Mao, S Manfredo-Vieira, J Lee, D Patel… - Nature …, 2023 - nature.com
… pathophysiology, including rare anti-MuSK B cells and polyclonal … T cells were detected in
spleen and lymph nodes with … labeling, T cells were labeled with CTV Cell Proliferation Kit (…

[HTML][HTML] Nuclear antigen–reactive CD4+ T cells expand in active systemic lupus erythematosus, produce effector cytokines, and invade the kidneys

…, U Stervbo, N Babel, C Meisel, T Alexander… - Kidney International, 2021 - Elsevier
… the detection of human autoantigen specific CD4 + T cells … of autoreactive T cells in SLE,
primarily using proliferation, … highly sensitive techniques as T-cell libraries or ARTE methods

[HTML][HTML] Antigen-specificity measurements are the key to understanding T cell responses

R Tippalagama, LY Chihab, K Kearns, S Lewis… - Frontiers in …, 2023 - frontiersin.org
proliferation and differentiation of antigen-specific effector … ie, αβ T cells or more generally
referred to as just T cells. The α/β … with isolation of rare antigen-binding precursor T cells (121), …

High-Throughput Detection of Autoantigen-Specific B Cells Among Distinct Functional Subsets in Autoimmune Donors

SA Smith, DJ Moore, LJ Crofford… - B Cell Activation and …, 2022 - books.google.com
T cells to drive their activation, proliferation, and effector cell differentiation and/or by differentiating
into autoantibody-secreting cells… repertoire that are as rare as 1 in 20 million cells (29)…

Identification of human antigen-specific CD4+ cells with peptide-MHC multimer technologies

IT Chow, WW Kwok - T-Helper Cells: Methods and Protocols, 2021 - Springer
… CD4 T cells with low frequency in peripheral blood, detection can … and T cell proliferation
assay of single cell-sorted T cell … sensitive than the corresponding tetramers in the detection of …

[HTML][HTML] Effects of alemtuzumab on (Auto) antigen-specific immune responses

C Hilger, C Riedhammer, E Orsó… - Frontiers in …, 2020 - frontiersin.org
cells in relation to the number of present CD4+ T cells, we … , the T lymphocytes had a normal
proliferation and cytokine … organs for detection of autoantigen-specific T cell responses. …

[HTML][HTML] Methods to Assess Proliferation of Stimulated Human Lymphocytes In Vitro: A Narrative Review

N Ganesan, S Ronsmans, P Hoet - Cells, 2023 - mdpi.com
sensitive as a marker of proliferation for the detection of raresensitive marker, since its
expression identified almost two times the frequency of proliferating T cells than was detected by …