Stabilities and instabilities in transgene expression

P Meyer - Transgenic plant research, 2022 - taylorfrancis.com
… to the presence of multiple copies of the transgene or homologous … its transcription, or by
post transcriptional processes that … High frequency, heat treatment-induced inactivation of the …

Transcriptional and post-transcriptional controls for tuning gene expression in plants

V Zhong, BN Archibald, JAN Brophy - Current Opinion in Plant Biology, 2023 - Elsevier
… In contrast, histone methylation is typically associated with transcriptional repression. To …
: multiple native introns were shown to mediate post-transcriptional enhancement of transgene

Gene regulation at transcriptional and posttranscriptional levels to combat salt stress in plants

A Singh, A Roychoudhury - Physiologia Plantarum, 2021 - Wiley Online Library
… In recent times, genetic engineering has been largely followed to confer salt tolerance
through introgressions of single transgenes or stacking multiple transgenes. However, most of …

[PDF][PDF] The sound of silence: Transgene silencing in mammalian cell engineering

A Cabrera, HI Edelstein, F Glykofrydis, KS Love… - Cell systems, 2022 - cell.com
… formation, DNA methylation, and posttranslational modifications (… or characterization of
multiple DNA elements (promoter, … terminal repeats act as targets for transcriptional repression. …

Application of linked and unlinked co-transformation to generate triple stack, marker-free, transgenic white clover (Trifolium repens L.)

R Narancio, YL Ding, YH Lin, S Sahab, S Panter… - Plant Cell, Tissue and …, 2020 - Springer
transgene transcription levels may be restored by removal of the T-DNA carrying the
CaMV35S:GFP transgene … In conclusion, insertion of a large T-DNA for stacking multiple

[HTML][HTML] Plant DNA Repair and Agrobacterium T−DNA Integration

SB Gelvin - International journal of molecular sciences, 2021 - mdpi.com
… individual transgenic plants each harboring a different T-DNA … that multiple DNA repair
pathways may contribute to T-DNA … 3′ end of the broken plant DNA following VirD2 removal. (C) …

[PDF][PDF] Regulation of rice tillering by RNA-directed DNA methylation at miniature inverted-repeat transposable elements

L Xu, K Yuan, M Yuan, X Meng, M Chen, J Wu, J Li… - Molecular plant, 2020 - cell.com
… In this study, we find that RdDM suppresses rice tillering. One … tillering not through
transcriptional repression or activation of … All of the transgenic plants generated in this study are in …

[HTML][HTML] Introns mediate post-transcriptional enhancement of nuclear gene expression in the green microalga Chlamydomonas reinhardtii

T Baier, N Jacobebbinghaus, A Einhaus… - PLoS …, 2020 - journals.plos.org
… efficiency from removal of E1 and … transgene DNA into the nuclear genome, individual
transformants contain different chromosomal integration sites and potentially underwent multiple

Non-perfectly matching small RNAs can induce stable and heritable epigenetic modifications and can be used as molecular markers to trace the origin and fate of …

Y Fei, T Nyikó, A Molnar - Nucleic Acids Research, 2021 - academic.oup.com
transgene-derived sRNAs due to transgeneRepression of FWA is associated with early
flowering phenotype. In the fwa-d epigenetic mutant, DNA methylation at direct tandem repeats

[HTML][HTML] Insights into genetic and molecular elements for transgenic crop development

MF Basso, FBM Arraes, M Grossi-de-Sa… - Frontiers in Plant …, 2020 - frontiersin.org
high efficiency in DNA excision. In addition to the elimination of the CRISPR/Cas9 transgene
by … ZNFs consist of two modules of tandem repeat DNA-binding domains flanking the FokI …