[HTML][HTML] Genetically encoded biosensors for evaluating NAD+/NADH ratio in cytosolic and mitochondrial compartments

Q Hu, D Wu, M Walker, P Wang, R Tian, W Wang - Cell reports methods, 2021 - cell.com
… spectrometry analysis have been used to monitor whole-cellcell membrane or mitochondrial
inner membrane in H9C2 … glucose only for bioenergetics studies in cardiomyocytes …

Role of mitochondrial membrane potential and lactate dehydrogenase a in apoptosis

S Zaib, A Hayyat, N Ali, A Gul… - Anti-Cancer Agents in …, 2022 - ingentaconnect.com
… In the Warburg effect, cancer cells prefer to undergo … degradation of mitochondrial
bioenergetics processes [147]. … of H9c2 cardiac myoblast cells exposed to 2-deoxyglucose-induced …

Concurrent Subcellular Delivery of Hydrogen Sulfide and a Payload with Near-Infrared Light

K Hanc, H Janeková, P Štacko - JACS Au, 2024 - ACS Publications
… % MeOH or 5% MeCN determined by methylene blue assay. … photouncaging using the
established methylene blue (MB) assay (… To ameliorate this, we performed the MB analysis using …

Physiological roles of hydrogen sulfide in mammalian cells, tissues, and organs

G Cirino, C Szabo… - Physiological …, 2023 - journals.physiology.org
… or sulfhydration) of proteins and the effect of H 2 S on various … and the regulation of cellular
bioenergetics and metabolism. The … The most widely used method, the methylene blue assay (…

The potential of the novel NAD+ supplementing agent, SNH6, as a therapeutic strategy for the treatment of Friedreich's ataxia

S Chiang, DS Kalinowski, M Dharmasivam… - Pharmacological …, 2020 - Elsevier
bioenergetics. SNH6 also chelated iron in cultured cardiac … Notably, the left ventricle (LV) of
the heart was used for analysis … on 59 Fe mobilization from H9c2 cells was determined by 59 …

[HTML][HTML] SMYD1a protects the heart from ischemic injury by regulating OPA1-mediated cristae remodeling and supercomplex formation

MW Szulik, S Valdez, M Walsh, K Davis, R Bia… - Basic Research in …, 2023 - Springer
… the effects of SMYD1a overexpression on the mitochondrial function we performed a Cell
Mito Stress Test in H9c2 … Quantitative analysis (D, F) of mitochondrial OCR from H9c2 cells

[HTML][HTML] Excess protein O-GlcNAcylation links metabolic derangements to right ventricular dysfunction in pulmonary arterial hypertension

SZ Prisco, L Rose, F Potus, L Tian, D Wu… - International journal of …, 2020 - mdpi.com
… , we conducted cellular fractionation experiments. MCT … effect of colchicine on mitochondrial
function and O-GlcNAcylation, we performed Seahorse microplanimetry analysis on H9c2

Modulating Cytotoxicity with Lego-like Chemistry: Upgrading Mitochondriotropic Antioxidants with Prototypical Cationic Carrier Bricks

S Benfeito, C Fernandes, D Chavarria… - Journal of Medicinal …, 2023 - ACS Publications
cells and HepG2 cells and improved cellular bioenergetics in the former, we performed
additional assays to understand the effects … The analysis of cellular bioenergetics was evaluated …

Bisphenol a (BPA) exposure aggravates hepatic oxidative stress and inflammatory response under hypertensive milieu–impact of low dose on hepatocytes and …

M Nagarajan, GB Maadurshni, J Manivannan - Food and Chemical …, 2024 - Elsevier
… To assess the statistical significance between means of values observed from the cellular
parameters, the current study utilized a one-way analysis of variance (ANOVA) followed by a …

[HTML][HTML] Targeting Energy Protection as a Novel Strategy to Disclose Di'ao Xinxuekang against the Cardiotoxicity Caused by Doxorubicin

T Wang, C Yuan, J Liu, L Deng, W Li, J He… - International Journal of …, 2023 - mdpi.com
… times that in plasma; induced bioenergy disorder is one of the … survival rate of H9c2 cells (rat
heart myoblast cell line) and … myocardial cell damage, we conducted enrichment analysis of …