… is inherently more flexible, which makes some well-established techniques for structural
determination of proteins, such as X-ray crystallography (XRC) and cryo-electron microscopy (…

… problem associated with spin-labeling by chemical synthesis of … label Çm into RNA with
quantitative spin-labeling efficiency. … and their spin labeling efficiency was determined by spin-…

… in nitroxide spin-labeling of nucleic acids for structure determination using rigid … spin-labeling
of duplex DNA and RNA containing abasic sites. No specific binding was observed for RNA…

… Depending on the desired positions for spin labeling within the RNA sequence of interest, …
Determine the concentration of cleaned spin-labeled RNA by measuring the absorption at …

… used to determine the structural properties of deoxyribonucleic acid (DNA), ribonucleic acid
(… In this work, we performed extensive MD simulations of RNA structures with either N T (N = …

… Moreover, it sets the path to label, for example, long riboswitches, ribozymes, and
DNAzymes for coarse-grained structure determination and enables to investigate mechanistical …

… DPAGE, the spin-labeling efficiency was determined (Figure … DNA and RNA, respectively,
with high spin-labeling efficiency… are nonperturbing of duplex structures. These new rigid spin …

… Efforts to obtain well-diffracting crystals of the 5 U analogue duplex 5 were unsuccessful;
however, we could determine the structure of the related single spin-labelled duplex 7 (Figure …

… active esters was utilized for RNA site-specific spin labeling with nitroxide radicals for EPR
… To determine the effect of hydantoin structure on the biological activity of tRNAs, model 17-…

… by a section on site-directed spin labeling of RNA, and then several examples are summarized
… Base-specific spin-labeling of RNA for structure determination. Nucleic Acids Res. 35 (9): …