Biological method has evolved to become an important field of nano-biotechnology due to its harmless nature, to be fast and cost-effective. Silver nanoparticles (SNPs) have been the research topic for their unique properties such as diagnosing, treating, and preventing various diseases in all aspects of human life. This work aimed to establish an extracellular and intracellular synthesis of SNPs from Fusarium oxysporum, focusing on evaluating their technological potential. The SNPs thus synthesized were visually characterized by the change of colour then confirmed by Ultra-violet (UV) Visible spectroscopy. The evaluation of the antimicrobial activity against Proteus mirabilis; Streptococcus bovis; Staphylococcus epidermidis; S. aureus; Salmonella typhi; Escherichia. coli and Candida albicans showed a very effective inhibitory with 18±0.66mm as a highest value. The antioxidant activity was tested using the DPPH method, and the synthesized nanoparticles recorded a remarkable percentage of free radical scavenges at 82,12 ± 0,42%, 70,46 ± 1,53% and 72,65 ± 1,33% for aqueous fungal extract, cell filtrate and biomass, respectively. The ability of the SNPs to detect hydrogen peroxide was illustrated by discoloration of the synthetic mixture, then confirmed by decreasing towards the disappearance of the characteristic peak. Finally, the photocatalytic performance was studied by the degradation of methylene blue. This activity showed a very interesting decrease in the peak intensity characteristic of this dye. In conclusion, synthesizing SNPs using F. oxysporium has proved their important technological property for the biological activities investigated.