Modulation of phosphatidylinositol 3-kinase activity during in vitro oocyte maturation increases the production of bovine blastocysts

JL Pereira, AG Curcio, LM Barroso… - Zygote, 2020 - cambridge.org
… Temporal inhibition of meiosis resumption has been used as a … Bovine oocyte meiotic
inhibition before in vitro maturation … stage for 24 hours in culture by specific inhibition of MPF …

The use of adenosine to inhibit oocyte meiotic resumption in Bos taurus during pre-IVM and its potential to improve oocyte competence

J Caballero, P Blondin, C Vigneault, MA Sirard… - Theriogenology, 2020 - Elsevier
… of blastocysts. The results showed the efficacy of ADO to … Bovine oocytes used for in vitro
maturation (IVM) are mainly from … Oocytes denuded of their cumulus cells were stored for 24 h in …

… and tensin homologue (PI3K/AKT/PTEN) pathway inhibitors during in vitro maturation of mammalian oocytes on in vitro embryo production: A systematic review

LP Alcaráz, L Prellwitz, G Alves, JMG Souza-Fabjan… - Theriogenology, 2022 - Elsevier
inhibition during in vitro maturation on meiosis resumption … 4) supplemented the LY Inhibitor
during bovine oocyte IVM. … or delays the GVBD in the first 24 h of IVM. Also, according to …

Approaches to oocyte meiotic arrest in vitro and impact on oocyte developmental competence

D Richani, RB Gilchrist - Biology of Reproduction, 2022 - academic.oup.com
inhibition completely prevents in vitro meiotic resumption of mouse and rat oocytes [29, 42],
whilst in bovine oocytes, PDE3 inhibition … hMG administration benefited from 24 h of CNP-pre-…

Epigenetic modifiers during in vitro maturation as a strategy to increase oocyte competence in bovine

NZ Saraiva, CS Oliveira, NNC Almeida, MR Figueiró… - Theriogenology, 2022 - Elsevier
… Samples were obtained after 20 h, 24 h and 28 h of IVM, and … embryo development after
in vitro fertilization of oocytes … (HDACs) inhibitor, during in vitro maturation of bovine oocytes, …

The SPOM-adapted IVM system improves in vitro production of bovine embryos

GR Leal, MAG Graciosa, CAS Monteiro, R Pasolini… - Theriogenology, 2020 - Elsevier
… -oocyte cAMP levels decrease and meiotic resumption begins non-physiologically, attributed
to the removal of inhibitory … that the reduction from 28 to 24 h in the duration of SPOM IVM …

Activation of bovine oocytes by protein synthesis inhibitors: new findings on the role of MPF/MAPKs

C Valencia, FA Pérez, C Matus, R Felmer… - Biology of …, 2021 - academic.oup.com
… synthesis inhibitor, in the in vitro production of bovine embryos up to the … After 24 h of in
vitro maturation, the oocytes were stripped of cumulus cells via vortex for 5 min in the HH embryo

In vitro culture strategy for oocytes from early antral follicle in cattle

RG Barros, V Lodde, F Franciosi… - JoVE (Journal of Visualized …, 2020 - jove.com
… 3 inhibitor was used to prevent untimely meiotic resumption … In vitro embryo production (IVP)
technologies have attempted to … in our lab where the oocytes were cultured for up to 24 h 2 . …

[HTML][HTML] In vitro maturation in the presence of Leukemia Inhibitory Factor modulates gene and miRNA expression in bovine oocytes and embryos

M Vendrell-Flotats, T García-Martínez… - Scientific reports, 2020 - nature.com
… transcripts are used for meiosis resumption and during the … in expanded blastocysts arising
from bovine oocytes in vitroAfter 24 h of IVM, oocytes were in vitro fertilized and cultured. …

[HTML][HTML] Histone deacetylase inhibitor during in vitro maturation decreases developmental capacity of bovine oocytes

TP Pontelo, MM Franco, TS Kawamoto, FMC Caixeta… - Plos one, 2021 - journals.plos.org
… the oocyte developmental competence, embryo production and … scriptaid did not affect
embryo production on D7. However, … resumption of meiosis is not necessary for acquiring oocyte