[HTML][HTML] Bacterial genetic engineering by means of recombineering for reverse genetics

U Fels, K Gevaert, P Van Damme - Frontiers in Microbiology, 2020 - frontiersin.org
… a double strand break (DSB) in the chromosome. This way, either the bacteria cannot survive…
Another way of increasing the percentage of edited cells in the outgrowth plate is by simply …

[HTML][HTML] Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics

S Qin, W Xiao, C Zhou, Q Pu, X Deng, L Lan… - Signal transduction and …, 2022 - nature.com
… secretion system directly secretes proteins from bacterial cytosol to … system are first exported
to the periplasm through the Sec or Tat system, and then crossing outer membranes through

Translocation of proteins through a distorted lipid bilayer

X Wu, TA Rapoport - Trends in cell biology, 2021 - cell.com
… classic Sec system in which translocation occurs through an … include membrane protein
insertion by YidC in bacteria and … into lipid, and only the other part of the hairpin is translocated …

[HTML][HTML] HtrA family proteases of bacterial pathogens: pros and cons for their therapeutic use

RY Xue, C Liu, QT Xiao, S Sun, QM Zou… - Clinical Microbiology and …, 2021 - Elsevier
… family, containing Sec exit signal peptide [7], … break through the epithelial barrier because
HtrA can also cleave fibronectin, occludin and claudin-8 as well as extracellular matrix proteins

Signal peptide efficiency: from high-throughput data to prediction and explanation

S Grasso, V Dabene, MMWB Hendriks… - ACS synthetic …, 2023 - ACS Publications
… defines the N-terminus of the exported protein and is essential for its … bacterial glycerol
stocks, fluorescein–starch and sodium alginate, which was processed through a laminar jet break-…

Bacterial outer membrane vesicles as a versatile tool in vaccine research and the fight against antimicrobial resistance

Z Zhu, F Antenucci, KR Villumsen, AM Bojesen - MBio, 2021 - Am Soc Microbiol
… the breakage of links between the bacterial … (SEC) isolates OMVs by trapping them into
nano-sized pores in porous resin particles to delay their elution time to achieve purification. SEC

SynBio and the boundaries between functional and pathogenic RepA-WH1 bacterial amyloids

R Giraldo - Msystems, 2020 - Am Soc Microbiol
… , and FapF as the export channel (10, 34). In summary, … β-sheet dimerization interface by
breaking the network of cation-… with higher affinity of the other half of each iteron by WH2, the C-…

[HTML][HTML] The biogenesis of β-lactamase enzymes

N Kaderabkova, M Bharathwaj, RCD Furniss… - …, 2022 - microbiologyresearch.org
… Narrow-spectrum β-lactamases can only break down penicillins and early (first- and second-)
… in the ‘export’ column, the majority of enzymes are translocated through the Sec system, …

Unifying themes and distinct features of carbon and nitrogen assimilation by polysaccharide-degrading bacteria: a summary of four model systems

JG Gardner, HJ Schreier - Applied Microbiology and Biotechnology, 2021 - Springer
bacteria that have established themselves as model systems … nutrient transport strategies
across different environments. A … this bacterium uses the Sec system for protein export similar …

Architecture of the membrane-bound cytochrome c heme lyase CcmF

A Brausemann, L Zhang, L Ilcu, O Einsle - Nature chemical biology, 2021 - nature.com
… By contrast, the bacterial and archaeal systems are typically … , the apocytochrome chain is
exported via the Sec system. Its … 2+ state was suggested to facilitate the breaking of this bond in …