… in the chemical, enzymatic and chemoenzymatic preparation of unnatural sugar nucleotides,
in … In addition, bioorthogonal labeling using unnatural sugar nucleotides catalyzed by …

… drawback of this approach (unless unnatural base pairs are used)… the development of
chemo-enzymatic RNA labeling methods … preferred labeling sites on ribonucleosides for …

… While limited proof of concept examples for cell-based applications of MT-enabled bioorthogonal
labeling/conjugation exist, three key barriers to broader use remain. First, unlike many …

… engineering and chemoenzymatic glycan labelling, focusing … or enzymatic oxidation of
glycans enables the labelling of … of the ensuing bioorthogonal labelling reaction. Comparative …

… In this way, researchers can design transgenic organisms in which the desired cell types
possess the appropriate enzymes for bioorthogonal labeling so that when the …

… of enzymes with relaxed substrate specificity permit access to unnatural sugar nucleotides,
required for furthering our understanding and control of chemoenzymatic glycan synthesis. …

… The chemoenzymatic method transfers monosaccharide analogues in the form of nucleotide
sugar … that are tolerable to unnatural sugar donors and specific to the glycan acceptor. …

… bioorthogonal reactions. On the other hand, the number of … , followed by chemo-enzymatic
strategies and ribozymes, and … covers enzymatic strategies for direct post-synthetic labeling …

… enzyme (FGE) create aldehydes on the oligosaccharide and cysteine residues, respectively,
which can selectively be labeled with a broad range of bioorthogonal … introduce non-natural …

… nucleotide as the donor substrates has made it possible to incorporate a handle selectively
at the antibody’s glycans for subsequent bioorthogonal … sugar nucleotide and enzymes and …