… This includes altering DNA looping in the promoter region, … protein-DNA composite during
Tn10/IS10 transposition (39). Our … Then, the chloramphenicol resistance (cat) gene, flanking by …

… Based on selected deletions and phenotypic analyses we … chlamydial promoter and a modified
transposon bearing the RSGFP-cat … RSGFP is contiguous with the chloramphenicol acetyl …

… toxic genes used over the years in positive-selection vectors. … chloramphenicol
acetyltransferase (encoded by the cat gene), … is encoded by a 1102-bp segment of transposon …

… basis of ICEs transposition, target site selection and how these … In this work, I established
in vivo transposition systems to … sequence play essential roles in Tn5397 target site selection. …

… low or no sequence specificity in their target selection, some … is usually tightly regulated
to ensure low transposition … BL21(DE3)-Δhfq::cat S1, which is an Hfq-null derivative of the …

… -15 to a resident plasmid and transpose to another strain and … Figure 5.7: ampC PCR
products from selected isolates with … of the bottle) of chloramphenicol and the glycopeptides and …

… Some of the most widely used universal bacterial plasmids … options for antibiotic selection
markers (chloramphenicol, ampicillin… , catI, and chloramphenicol promoter (cat promoter) were …

… are promoterless, while the cat cassette was subcloned with its own constitutive … Thus,
transposon mutagenesis of res-cassette containing strains following chloramphenicol selection, …

… the most widely used methods are the mariner transposon-… , transposon mutants are
enriched through liquid selection, … , transposon insertions in regulatory regions (eg, promoters), …

… expression of their IS50 and IS10 transposases at the post-… /2L, the chloramphenicol-resistant
gene CAT (chloramphenicol … transposition without affecting the selection of transposition …