… antibiotic that covalently modifies an active site cysteine of UDP-N-… The aglE neighborhood
cloned and expressed by the … Next, we searched public sequence databases for additional …

3-Hydroxyarginine (3-OH-Arg) is an important intermediate for the synthesis of viomycin, an
important antibiotic for the clinical treatment of tuberculosis. An efficient strategy for 3-OH-Arg …

… ’ nucleotide sequences were verified by digesting the cloned … the most popular expression
plasmid, and it contains the T7 … for cloning purposes, as these restriction enzymes sites were …

… the active site (Chaturvedi et al., 2021). Nevertheless, the 5hydroxyarginine product decomposes
… A QM region was selected that contains the metal and its first-coordination sphere, the …

… of L-arginine resulted in LN-hydroxyarginine (NOHA) [10], … and sub-cloned into pETM11
expression plasmid previously … site at the N-terminus as confirmed by Sanger sequencing. …

… Using genome sequence information with the transcriptomic data, we … contained genes
encoding known fps and collagens, we listed genes exclusively expressed in the foot, the site of …

… to produce 5-hydroxy-arginine. This finding identifies 2-ODD-… Ava5009 from Anabaena
variabilis were cloned in pET24 for … fusion site causes a frame shift and inactivates both genes. A …

… was sequenced and analyzed, and the biosynthetic gene … the pentapeptides; however, it
contains a stand-alone PBP-type … Cloning and heterologous expression of the cpp cluster ..........…

… Pns, and N ε -hydroxyarginine, a component among small … The pseudomonad pathway
contains two genes (psf3 and … showed that several active site residues fix the orientation of …

… , we are displaying the site without styles and JavaScript. … of 3, which contains two amino
acids, N 5 -hydroxyarginine, and … Then we cloned and overexpressed alpG in E. coli with an N-…