[HTML][HTML] SIMPLE 3.0. Stream single-particle cryo-EM analysis in real time

J Caesar, CF Reboul, C Machello, S Kiesewetter… - Journal of structural …, 2020 - Elsevier
… obtain a clean SP data set amenable to high-resolution 3D … (process #2, executed after #1
stream pre-processing), the viewer … for on-the-fly electron-microscopy single-particle image …

TranSPHIRE: automated and feedback-optimized on-the-fly processing for cryo-EM

M Stabrin, F Schoenfeld, T Wagner, S Pospich… - Nature …, 2020 - nature.com
… offers reliable on-the-fly processing, resulting in high-resolutionsingle particle analysis
pipeline, but it lacks any automatic … a deep learning-based pre-processing routine that can filter …

New tools for automated cryo-EM single-particle analysis in RELION-4.0

D Kimanius, L Dong, G Sharov, T Nakane… - Biochemical …, 2021 - portlandpress.com
… was calculated over the entire data set, η can now be set to 1.0, … To simplify launching of
on-the-fly image processing and … .py functionality by linking microscope data acquisition with the …

AutoEMage: automatic data transfer, preprocessing, real-time display and monitoring in cryo-EM

Y Cheng, X Huang, B Xu, W Ding - Journal of Applied …, 2023 - journals.iucr.org
… (cryo-EM), especially single-particle analysis, has become a … , ie data transfer and file backup,
and pre-processing tasks, ie … With access to these processed results ‘on-the-fly’, users can …

nextPYP: a comprehensive and scalable platform for characterizing protein variability in situ using single-particle cryo-electron tomography

HF Liu, Y Zhou, Q Huang, J Piland, W Jin, J Mandel… - Nature …, 2023 - nature.com
single-particle cryo-electron tomography data analysis from on-the-fly pre-processing of
tilt series to high-resolution … during data collection and includes the steps of frame and tilt-series …

Automated pipelines for rapid evaluation during cryoEM data acquisition

JH Mendez, EYD Chua, M Paraan, CS Potter… - Current Opinion in …, 2023 - Elsevier
single particle analysis, has experienced an increase in popularity among researchers
interested in determining high-resolution … to handle on-the-fly pre-processing on data collection

IceBreaker: Software for high-resolution single-particle cryo-EM with non-uniform ice

M Olek, K Cowtan, D Webb, Y Chaban, P Zhang - Structure, 2022 - cell.com
on-the-fly processing to automatically pick as many particles as possible from each micrograph
and to select optimal regions for data collection. … The pre-processing stage includes low-…

Single particle cryo-electron microscopy: from sample to structure

JBR White, DP Maskell, A Howe, M Harrow… - JoVE (Journal of …, 2021 - jove.com
… Once a suitable grid has been identified, data collection can … are optimized to collect
high-resolution data in an automated … facilities offer pre-processing of micrograph movies 'on the

Single-particle cryo-EM data collection with stage tilt using leginon

S Aiyer, TS Strutzenberg, ME Bowman, JP Noel… - JoVE (Journal of …, 2022 - jove.com
high-resolution structures of macromolecules and macromolecular assemblies solved using
… ) has been utilized for pre-processing. On-the-fly data processing provides an initial cryo-EM …

CASSPER is a semantic segmentation-based particle picking algorithm for single-particle cryo-electron microscopy

B George, A Assaiya, RJ Roy, A Kembhavi… - Communications …, 2021 - nature.com
… particles on-the-fly, enabling data processing automation. … of CASSPER in obtaining
high-resolution 3D reconstruction, we … The pre-processing and deep learning tools were …