Porcine oocyte preincubation in oviductal fluid flush before in vitro fertilization in the presence of oviductal epithelial cells improves monospermic zygote production

RITP Batista, LN Moro, E Corbin, C Alminana… - Zygote, 2021 - cambridge.org
… with heparin, an overall increase (P < 0.05) of the final output of … In conclusion, the combination
of treatment of oocytes by … of OEC, improved monospermic zygote production without …

[HTML][HTML] Oviduct fluid during IVF moderately modulates polyspermy in in vitro-produced goat embryos during the non-breeding season

GM Bragança, AS Alcântara-Neto, R Batista… - Theriogenology, 2021 - Elsevier
… supplemented with 5 μg/mL of heparin, 4 μg/mL gentamicin, … zygotes x 100); and IVF
efficiency (monospermic zygotes/total … pooled, gametes exposure to OF during IVF improved (P = …

Addition of exogenous proteins detected in oviductal secretions to in vitro culture medium does not improve the efficiency of in vitro fertilization in pigs

S García-Martínez, J Gadea, P Coy, R Romar - Theriogenology, 2020 - Elsevier
oviductal protein and in presence or absence of heparin to … ) effect showing that, together
with OVGP1, other candidate … or IVF yield (monospermic zygotes forming a male pronucleus …

[HTML][HTML] … of albumin by preovulatory oviductal fluid in swim-up sperm preparation method modifies boar sperm parameters and improves in vitro penetration of oocytes

S Navarro-Serna, E París-Oller, O Simonik, R Romar… - Animals, 2021 - mdpi.com
… to protect gametes from external harmful effects during in … in the medium did not improve
the sperm parameters results, … percentage of monospermic zygotes respect to total oocytes. …

Oviduct fluid extracellular vesicles regulate polyspermy during porcine in vitro fertilisation

AS Alcântara-Neto, M Fernandez-Rufete… - Reproduction …, 2020 - CSIRO Publishing
… of better IVF protocols that provide high monospermic … This will improve the production of
healthy embryos for the recovery of … However, the oviduct secretions are a complex mixture of …

Sperm migration, selection, survival, and fertilizing ability in the mammalian oviduct

C Mahé, AM Zlotkowska, K Reynaud… - Biology of …, 2021 - academic.oup.com
… Moreover, more than 30% of in vitro produced embryos may present … In cattle, heparin-like
GAGs are present in high abundance … In sheep, the penetration of oocytes and monospermic

In vitro production of small ruminant embryos: Latest improvements and further research

JMG Souza-Fabjan, RITP Batista… - Reproduction …, 2021 - CSIRO Publishing
… High concentrations of antioxidants or a mixture of them in IVM … We routinely use 10% ESS
with heparin (5μg mL À1 ) duringproduction of monospermic zygotes and tended to enhance

[HTML][HTML] Melatonin improves rate of monospermic fertilization and early embryo development in a bovine IVF system

JC Gutiérrez-Añez, H Henning, A Lucas-Hahn… - PloS one, 2021 - journals.plos.org
… Enhancing gametes and embryo competence during in vitro … μM hypotaurine, 1.0 IU/ml
heparin, and 1.0 μM epinephrine. … as a practical strategy to increase monospermic fertilization in …

[HTML][HTML] In vitro embryo production in small ruminants: what is still missing?

JMG Souza-Fabjan, GR Leal, CAS Monteiro… - Animal …, 2023 - SciELO Brasil
… in in vitro-produced goat embryos during the non-breeding season… the combination of ESS
(10%) and heparin (5 µg/mL) duringoviduct fluid on the production efficiency of monospermic

Effect of oviductal fluid on bull sperm functionality and fertility under non-capacitating and capacitating incubation conditions

N Küçük, JS Lopes, C Soriano-Úbeda, CO Hidalgo… - Theriogenology, 2020 - Elsevier
… glycoprotein 1 (OVGP1), osteopontin, heparin-like … embryos obtained from sperm cells
treated with oviductal … pronucleus formation and output (monospermic zygotes forming a male …