Nucleolar TFIIE plays a role in ribosomal biogenesis and performance

T Phan, P Maity, C Ludwig, L Streit… - … Acids Research, 2021 - academic.oup.com
… and quantified by using ImageJ. Antibodies used for western blot analysis are listed in …
Re-ChIP semi-quantitative analysis shows binding of TFIIE to the same rDNA molecules as …

[HTML][HTML] Challenges associated with homologous directed repair using CRISPR-Cas9 and TALEN to edit the DMD genetic mutation in canine Duchenne muscular …

S Mata López, C Balog-Alvarez, S Vitha, AK Bettis… - PloS one, 2020 - journals.plos.org
… Based on these results, methods must be modified to … with the Western blot results, perhaps
reflecting the semi-quantitative … myotubes measured with ImageJ and analyzed via one way …

Altitude acclimatization via hypoxia-mediated oxidative eustress involves interplay of protein nitrosylation and carbonylation: A redoxomics perspective

A Gangwar, S Paul, A Arya, Y Ahmad, K Bhargava - Life Sciences, 2022 - Elsevier
… were then immunoblotted (as described under Immunoblotting … , a protein with predicted
sites for both modifications but … be reached by the semi-quantitative analysis. Literature survey …

Alternative splicing of BCL-x is controlled by RBM25 binding to a G-quadruplex in BCL-x pre-mRNA

R Le Sénéchal, M Keruzoré, A Quillévéré… - … Acids Research, 2023 - academic.oup.com
… to GAPDH protein levels using the ImageJ software. … We first assessed, using semi-quantitative
RT–PCR, the … RT–PCR experiment is shown in the upper panel and western blot analysis

The prevalent NR2E3 c. 932G> A mutation induces aberrant splicing that can be rescued using splice-shifting antisense oligonucleotides

Y Sztainberg, M David Teitelbaum, I Buchumenski… - bioRxiv, 2024 - biorxiv.org
… further confirmed at the protein level by western blot analysis (Fig. … A semiquantitative
PCR mini screen was performed … ) and quantified by an ImageJ software package. Primary …

[HTML][HTML] DOT1L-mediated murine neuronal differentiation associates with H3K79me2 accumulation and preserves SOX2-enhancer accessibility

F Ferrari, L Arrigoni, H Franz, A Izzo, L Butenko… - Nature …, 2020 - nature.com
… limited by the use of semi-quantitative and low-throughput methods (eg, immunoblotting and
imaging). … For densitometric analyses, ImageJ software was used 55 . The code used for the …

[HTML][HTML] Cyclophilin A supports translation of intrinsically disordered proteins and affects haematopoietic stem cell ageing

L Maneix, P Iakova, CG Lee, SE Moree, X Lu… - Nature Cell …, 2024 - nature.com
… cells (HSPCs) through semi-quantitative two-dimensional (2D) gel … PPIA protein in HeLa
cells followed by a western blot to … stress granule analysis was carried out with ImageJ software. …

A recombinant signalling‐selective activated protein C that lacks anticoagulant activity is efficacious and safe in cutaneous wound preclinical models

R Zhao, M Xue, H Lin, M Smith, H Liang… - Wound Repair and …, 2024 - Wiley Online Library
… were collected for Western blot and gelatin zymography analysis respectively. Three wells …
Bands were quantified using ImageJ. … Qualitative analyses and semi-quantitative scoring of …

A small molecule potent IRAK4 inhibitor abrogates lipopolysaccharide-induced macrophage inflammation in-vitro and in-vivo

SA Choudhary, D Patra, A Sinha, S Mazumder… - European Journal of …, 2023 - Elsevier
… and was used as a loading control for semi-quantitative RT-PCR. The following cycling
conditions … Densitometric analysis of RT-PCR and Western blot data was performed using ImageJ

Simvastatin and Lovastatin inhibit Bacillus subtilis biofilm formation by interfering with the aggregation and amyloid formation of the TasA (28-261)–TapA (33-253) …

N Verma, M Bajia, AK Yadav, C Chaudhary, D Meena… - 2024 - researchsquare.com
Semi-quantitative RT-PCR analyses of major biofilm-associated genes 6 The primers for
Semi-quantitative RT-PCR analyses were designed by using the … to multiple computational tools …