… For a subset of these cassettes, we studied the impact of target position, Cas9 expression …
cleaved chromosome using native homologous recombination, the cell dies. Therefore, Cas9:…

… Upon cleavage by Cas9, in the absence of an appropriate repair … genes was tuned and
chromosomal targets in virulent, E. … effect including experimental off-target cleavage validation, …

… /Cas9 to generate a DSB and induce intra-chromosomal … regions with no off-target effects,
and edit large areas of up to … different efficiencies in the cleavage of complementary and non-…

… the unedited 5′ flap, only the cleavage of the 5′ flap leads to … In summary, our results
indicate a very high fidelity of … coli, CRISPR-Cas9 has been widely employed as a tool for counter-…

… effects of genomic changes. We have found that a commonly used Cas9-assisted editing
method results … nt) spacer sequences to direct Cas9 cleavage were encoded on donor or guide …

… To understand the effects of ligand binding to the aptamer on the activity … cleavage of the
DNA target. To measure the endonuclease activity of the Cas9-gRNA complexes in vitro, Cas9 …

… The results showed that the ISApl1-CRISPR/Cas9 was … efficient ISApl1-CRISPR/Cas9
cleavage. The successful elimination … on their chromosomes, we sought to determine the impact of …

… Our results indicate that MCBAS carrying two gRNAs has better performance than that …
The MCBAS consist of CRISPR-Cas9 for cleavage of phage DNA and origin of transfer (oriT) for …

… The gRNA directs the Cas9 endonuclease to the cleavage … To reduce the off-target effects
of Cas9 in this study, it was … on the ETEC plasmids and chromosome for deleting, replacing, …

… effect of the CRISPR-Cas9 system of adaptive immunity on the prevention of the exogenous
resistant plasmids pNDM-5 by introducing the system into E coli … -Cas9 mediated cleavage …