Improving the activity and stability of Bacillus clausii alkaline protease using directed evolution and molecular dynamics simulation

J Li, L Jiang, X Cao, Y Wu, F Lu, F Liu, Y Li… - Enzyme and Microbial …, 2021 - Elsevier
… in detergent. Among them, alkaline protease accounts for a large share of detergent enzyme
sales… play an important role in reducing the cost of detergent enzymes. In our study, alkaline …

Detergent-free isolation of membrane proteins and strategies to study them in a near-native membrane environment

B Krishnarjuna, A Ramamoorthy - Biomolecules, 2022 - mdpi.com
detergents are avoided [67]. These observations suggest that detergents are not the ideal
systems for the characterization … may not be compatible with the functional analysis of various …

Foldable detergents for membrane protein study: Importance of detergent core flexibility in protein stabilization

L Ghani, S Kim, H Wang, HS Lee… - … A European Journal, 2022 - Wiley Online Library
… Many conventional detergents are efficient at protein … long enough for downstream
characterization. Because of their … , endowing detergent foldability, as supported by our MD …

Preparation, characterization, immobilization, and molecular docking analysis of a novel detergent-stable subtilisin-like serine protease from Streptomyces mutabilis …

S Mechri, F Allala, K Bouacem, I Hasnaoui… - International Journal of …, 2022 - Elsevier
… The protein amount was quantified using the Pierce™ detergent compatible Bradford
assay kit purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). The …

Rationalizing the optimization of detergents for membrane protein purification

LH Urner, F Junge, F Fiorentino… - … A European Journal, 2023 - Wiley Online Library
… gas phase and are compatible to condensed-phase methods (Figure 1a). 3b, 8 In solution, …
will facilitate the delivery of new detergent tools for the analysis of challenging drug targets. …

[HTML][HTML] A 10-year meta-analysis of membrane protein structural biology: detergents, membrane mimetics, and structure determination techniques

BC Choy, RJ Cater, F Mancia, EE Pryor Jr - Biochimica et Biophysica Acta …, 2021 - Elsevier
… Alternatively, amphipols and lipid nanodiscs – while not compatible with crystallographic
analysis – carry several benefits for structure determination using cryo-EM and, coincidently, …

A broad temperature active lipase purified from a psychrotrophic bacterium of sikkim himalaya with potential application in detergent formulation

A Kumar, S Mukhia, N Kumar, V Acharya… - … in bioengineering and …, 2020 - frontiersin.org
… Bioinformatics analysis also supported in vitro findings by predicting enzyme's broad … The
compatibility of the purified lipase with regular commercial detergents, coupled with its versatile …

Temporal and thermal dynamics exploration of different detergents' formulations components on fungal alkaliphilic lipases stability

H Majeed, T Iftikhar, K Maqsood - Zeitschrift für Physikalische …, 2024 - degruyter.com
… and compatibility with other detergent components [5]. Aspergillus niger lipases have been
reported to be effective in laundry detergents… , Tween 80, and commercial detergents [6]. The …

Conformationally flexible core-bearing detergents with a hydrophobic or hydrophilic pendant: Effect of pendant polarity on detergent conformation and membrane …

A Sadaf, S Kim, HE Bae, H Wang, A Nygaard, Y Uegaki… - Acta biomaterialia, 2021 - Elsevier
… current study reveals the effect of detergent pendant polarity on … Such an analysis is essential
for future effective detergent … , detergent chain length is crucial for compatibility of detergent

Nonionic detergent micelle aggregates: An economical alternative to protein A chromatography

G Dhandapani, E Wachtel, M Sheves, G Patchornik - New biotechnology, 2021 - Elsevier
Characterization of detergent aggregates for purifying hIgG … Dynamic light scattering (DLS)
analysis of hIgG purified via … , continuous production flows is supported by the findings that: (i) …