Improving the activity and stability of Bacillus clausii alkaline protease using directed evolution and molecular dynamics simulation
J Li, L Jiang, X Cao, Y Wu, F Lu, F Liu, Y Li… - Enzyme and Microbial …, 2021 - Elsevier
… in detergent. Among them, alkaline protease accounts for a large share of detergent enzyme
sales… play an important role in reducing the cost of detergent enzymes. In our study, alkaline …
sales… play an important role in reducing the cost of detergent enzymes. In our study, alkaline …
Detergent-free isolation of membrane proteins and strategies to study them in a near-native membrane environment
B Krishnarjuna, A Ramamoorthy - Biomolecules, 2022 - mdpi.com
… detergents are avoided [67]. These observations suggest that detergents are not the ideal
systems for the characterization … may not be compatible with the functional analysis of various …
systems for the characterization … may not be compatible with the functional analysis of various …
Foldable detergents for membrane protein study: Importance of detergent core flexibility in protein stabilization
… Many conventional detergents are efficient at protein … long enough for downstream
characterization. Because of their … , endowing detergent foldability, as supported by our MD …
characterization. Because of their … , endowing detergent foldability, as supported by our MD …
Preparation, characterization, immobilization, and molecular docking analysis of a novel detergent-stable subtilisin-like serine protease from Streptomyces mutabilis …
… The protein amount was quantified using the Pierce™ detergent compatible Bradford
assay kit purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). The …
assay kit purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). The …
Rationalizing the optimization of detergents for membrane protein purification
LH Urner, F Junge, F Fiorentino… - … A European Journal, 2023 - Wiley Online Library
… gas phase and are compatible to condensed-phase methods (Figure 1a). 3b, 8 In solution, …
will facilitate the delivery of new detergent tools for the analysis of challenging drug targets. …
will facilitate the delivery of new detergent tools for the analysis of challenging drug targets. …
[HTML][HTML] A 10-year meta-analysis of membrane protein structural biology: detergents, membrane mimetics, and structure determination techniques
BC Choy, RJ Cater, F Mancia, EE Pryor Jr - Biochimica et Biophysica Acta …, 2021 - Elsevier
… Alternatively, amphipols and lipid nanodiscs – while not compatible with crystallographic
analysis – carry several benefits for structure determination using cryo-EM and, coincidently, …
analysis – carry several benefits for structure determination using cryo-EM and, coincidently, …
A broad temperature active lipase purified from a psychrotrophic bacterium of sikkim himalaya with potential application in detergent formulation
… Bioinformatics analysis also supported in vitro findings by predicting enzyme's broad … The
compatibility of the purified lipase with regular commercial detergents, coupled with its versatile …
compatibility of the purified lipase with regular commercial detergents, coupled with its versatile …
Temporal and thermal dynamics exploration of different detergents' formulations components on fungal alkaliphilic lipases stability
H Majeed, T Iftikhar, K Maqsood - Zeitschrift für Physikalische …, 2024 - degruyter.com
… and compatibility with other detergent components [5]. Aspergillus niger lipases have been
reported to be effective in laundry detergents… , Tween 80, and commercial detergents [6]. The …
reported to be effective in laundry detergents… , Tween 80, and commercial detergents [6]. The …
Conformationally flexible core-bearing detergents with a hydrophobic or hydrophilic pendant: Effect of pendant polarity on detergent conformation and membrane …
… current study reveals the effect of detergent pendant polarity on … Such an analysis is essential
for future effective detergent … , detergent chain length is crucial for compatibility of detergent …
for future effective detergent … , detergent chain length is crucial for compatibility of detergent …
Nonionic detergent micelle aggregates: An economical alternative to protein A chromatography
G Dhandapani, E Wachtel, M Sheves, G Patchornik - New biotechnology, 2021 - Elsevier
… Characterization of detergent aggregates for purifying hIgG … Dynamic light scattering (DLS)
analysis of hIgG purified via … , continuous production flows is supported by the findings that: (i) …
analysis of hIgG purified via … , continuous production flows is supported by the findings that: (i) …