[HTML][HTML] Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma

CJC Rodrigues, CCCR de Carvalho - Frontiers in bioengineering and …, 2022 - frontiersin.org
… The presumption that one colony is the result of a single cell … plates and respective number
of colony forming units (CFU), … The coexistence of copiotrophic and oligotrophic bacteria in …

[HTML][HTML] A targeted liquid cultivation method for previously uncultured non-colony forming microbes

EY Seo, D Jung, SS Epstein, W Zhang… - Frontiers in …, 2023 - frontiersin.org
… Therefore, we assumed that a significant number of non-colony-forming bacteria exist in
sponge tissue and other marine environments, which partially explains the long-lasting problem …

[HTML][HTML] Tracking defined microbial communities by multicolor flow cytometry reveals tradeoffs between productivity and diversity

FS Midani, LA David - Frontiers in Microbiology, 2023 - frontiersin.org
… ecology using well-studied human bacteria. It can be extended … whether other species could
coexist with B. vulgatus at time … vulgatus followed by coexistence of various species (Figure 5…

Expanding success in the isolation of abundant marine bacteria after reduction in grazing and viral pressure and increase in nutrient availability

X Rey-Velasco, O Deulofeu-Capo… - Microbiology …, 2023 - Am Soc Microbiol
… We also show that top-down and bottom-up controls differentially affect taxa in terms of
culturability. Our results indicate that culturing marine bacteria using agar plates can be …

Spatial Structure Modulates Persister Formation in a Synthetic Cross-Feeding Bacterial Community

X Xiong - 2022 - search.proquest.com
coexistence between persister and growing cells. The … Most recently, single-cell, temporal
analysis of bacterial growth … by taking 100 µL for CFU (colony forming unit) counting and the …

The theoretical and experimental exploration of the use of predatory bacteria to control biofilms

A Ogundero - 2023 - theses.gla.ac.uk
… , in an open system and at the single cell level. All this can be … observed in the presence
of culturable prey and thus the … resistance development and the co-existence of predator and …

[HTML][HTML] Challenges of unculturable bacteria: environmental perspectives

A Bodor, N Bounedjoum, GE Vincze… - … Science and Bio …, 2020 - Springer
… as; metatranscriptomics, metabolomics, or even single-cell genomics, it can contribute to the
culturability. By now, many reports have revealed different physiological states in bacteria (…

[HTML][HTML] Enumeration of Viable Non-Culturable Vibrio cholerae Using Droplet Digital PCR Combined With Propidium Monoazide Treatment

S Zhao, J Zhang, Z Li, Y Han, B Kan - Frontiers in cellular and …, 2021 - frontiersin.org
Culturable cell counts during VBNC-state development of strain C6706. ∗ indicates that the
culturable cell count was <1 colony-forming … pathogens or other studies bacteria in samples, …

High-throughput single-cell phenotypic profiling and backtracing exposes and predicts clinically relevant subpopulations in isogenic Staphylococcus aureus …

J Hira, B Singh, T Halder, A Mahmutovic, C Ajayi… - bioRxiv, 2023 - biorxiv.org
… non-culturable (VBNC) was identified by observing a discrepancy between colony forming
units (… phenotypic profiling coupled to functional analysis of single bacteria (Figure 1). In a first …

[HTML][HTML] The Microbial Community Associated with Rhizostoma pulmo: Ecological Significance and Potential Consequences for Marine Organisms and Human …

L Stabili, L Rizzo, L Basso, M Marzano, B Fosso… - Marine drugs, 2020 - mdpi.com
… Each pooled compartment was then used for the 16S rDNA sequencing of the associated
microbial community [22], the estimation of culturable bacteria abundance (as colony forming