… Protease Inhibitor). The mixture samples were then centrifuged at 12,000g for 15 min at 4 C
after 30 min of ice … to the supernatant, and the proteins were precipitated overnight at −20 C. …

… Therefore, the aim of this study was to develop a simple and clear method to predict …
apoptotic cells, DNA-PKcs is cleaved by a CPP32-like apoptotic protease [20] or ICE-like protease …

… , as well as evaluating the implications of targeting DNA-PKcs … is the DNA-dependent protein
kinase catalytic subunit (DNA-… efficient apoptosis, which we further discuss in “Roles of DNA-…

… A caspase contains a nucleophilic cysteine in its active site that attacks a target protein after
… as a cysteine protease is the initiator of apoptosis process in C. elegans and CED-4 protein …

… adjacent motif (most likely off-targets) were identified in the Chinook … A sgRNA targeting the
mEGFP gene was also used as … with ice-cold Dulbecco’s PBS (DPBS), scraped in 1 ml of ice-…

… with Protease inhibitor cocktail (Thermo Fisher Scientific) on ice … response and p53
transcriptional targets among the DE … to an activator of p53-dependent apoptosis nutlin-3 [70] that …

… has emerged as a promising alternative therapeutic target. … strands of DNA, thereby
circumventing apoptosis induction. … 24 h under nitrogen gas and precipitated in ice-cold diethyl …

… interleukin-1β converting enzyme (ICE) [8]. Multiple … and a large and small catalytic subunit.
Initiator caspases have … and Rho-associated coiled coil containing protein kinase 1 (ROCK1). …

… apoptosis and was found to have significant homology to ICE. … anti-apoptotic transcription
factor and identified several target … The Ras-Raf-MEK-ERK, a Mitogen-activated protein kinase …

… with DNA-dependent protein kinase catalytic subunit (DNA-PKcs) … Given the critical role of
apoptosis in radioresistance, we … cells were trypsinized and washed with ice-cold PBS. Next, …