[HTML][HTML] Refining the transcriptome of the human malaria parasite Plasmodium falciparum using amplification-free RNA-seq

L Chappell, P Ross, L Orchard, TJ Russell, TD Otto… - BMC genomics, 2020 - Springer
… We also find that transcription from bidirectional promoters … by defining the position at which
continuous RNA-seq coverage … maximise the signal for defining this region. Like DAFT-seq, …

[HTML][HTML] A computational system for identifying operons based on RNA-seq data

B Tjaden - Methods, 2020 - Elsevier
… annotation of genes, transcription signals in the form of promoter regions or terminators,
codon … in a genome before determining which subset of directons are co-transcribed, ie, are …

[HTML][HTML] Systematic comparison and assessment of RNA-seq procedures for gene expression quantitative analysis

LA Corchete, EA Rojas, D Alonso-López… - Scientific reports, 2020 - nature.com
… gene expression and transcription activation at genome-… ) 55 , and 10 random genes from
the mid-area. Additionally, we … and false negative estimates were defined based on these qRT-…

[HTML][HTML] Handling multi-mapped reads in RNA-seq

G Deschamps-Francoeur, J Simoneau… - Computational and …, 2020 - Elsevier
… , use cellular RNAs as substrates, reverse transcribing and … of regions that are identical to
other genes but also regions … small RNA-seq where reads accumulate in very well defined

[HTML][HTML] Systematic assessment of long-read RNA-seq methods for transcript identification and quantification

FJ Pardo-Palacios, D Wang, F Reese, M Diekhans… - Nature …, 2024 - nature.com
… at transcription start sites (TSSs), transcription termination … non-spliced data and accurately
define TSSs and TTSs and the … regions, in which the length of the amplified region ranged …

[HTML][HTML] recount3: summaries and queries for large-scale RNA-seq expression and splicing

C Wilks, SC Zheng, FY Chen, R Charles, B Solomon… - Genome biology, 2021 - Springer
… , we also enable users to study transcription in any genomic region, annotated or not. …
Megadepth also summarizes the amount of sequencing coverage within genomic intervals defined

Systematic analysis of 1298 RNASeq samples and construction of a comprehensive soybean (Glycine max) expression atlas

FB Machado, KC Moharana, F Almeida‐Silva… - The Plant …, 2020 - Wiley Online Library
… Next, we applied a minimum TPM threshold of 1 to define a gene as expressed and found
that … We detected 6582 AS events, mostly transcription start sites and transcription termination …

[HTML][HTML] Detection of aberrant splicing events in RNA-seq data using FRASER

C Mertes, IF Scheller, VA Yépez, MH Çelik… - Nature …, 2021 - nature.com
… even stronger for highly transcribed introns (Supplementary Fig… region variants (“Methods”
section), which were defined as … site dinucleotide and splice region variants, as defined by the …

[HTML][HTML] iREAD: a tool for intron retention detection from RNA-seq data

HD Li, CC Funk, ND Price - BMC genomics, 2020 - Springer
… Another limitation of rMATS is that it focuses on determining … across the whole intron
region, a feature unique to iREAD. … because it could result from the transcription of exons. This …

[HTML][HTML] Intron retention as a mode for RNA-seq data analysis

JT Zheng, CX Lin, ZY Fang, HD Li - Frontiers in genetics, 2020 - frontiersin.org
… of truncated proteins translated from IRIs seem to be available in the existing literature. … It
is worth mentioning that rMATS adopts a flexible likelihood-ratio test, allowing users to define