… for crop improvement concerning adaptation, opportunities, and … Cas9 derived from
Staphylococcus aureus (SaCas9) and a … highly efficient method of the gene carrier for plant …

… aureus (SaCas9), is important in genome editing because it … an effective platform for multiplex
genome editing and also improves sgRNA expression level [23], so we choose to develop …

… ) to develop an efficient CRISPR-xCas9 genome editing system … adaptive immune systems
has facilitated diverse genomic … Several engineered SpCas9 and SaCas9 variants that can …

… PAM specificity of SaCas9 was relaxed to NNGRRT by SaCas9-KKH. These … One of the
most effective and promising applications of CBEs in plants has been development of herbicide …

… CRISPR/Cas9-mediated base-editing systems were developed to enable targeted nucleotide
… To date, two types of base-editing tools, cytidine base editor (CBE) and adenine base …

… repeat (CRISPR) RNA-guided Cas (CRISPR-associated sequence) genome-editing tools
are … To develop the SpCas9-NG tool with a decreased self-target effect, the sgRNA scaffold …

… ) from the type II CRISPR-Cas bacterial adaptive immune … makes them less popular tools in
genome engineering. Efforts to … develop an efficient FrCas9-based genome editing system in …

… and base editors are fundamental tools in plant genome editing. … Both SaCas9 and its KKH
variant have been shown to … and could be adapted for plant genome editing in the near future. …

… locus display characteristics preventing an efficient fixation of the CRISPR complex. For …
CRISPR-SaCas9 tools used and developed in this study broaden the scope of genome editing …

… plant genome editing applications. We consider the technical aspects of gRNA design and
the associated computational tools. … The rapid discovery and development of diverse CRISPR …