This is the first of two articles on the Extant Life Volumetric Imaging System (ELVIS)
describing a combined digital holographic microscope (DHM) and a fluorescence light-field
microscope (FLFM). The instrument is modular and robust enough for field use. Each mode
uses its own illumination source and camera, but both microscopes share a common
objective lens and sample viewing chamber. This allows correlative volumetric imaging in
amplitude, quantitative phase, and fluorescence modes. A detailed schematic and parts list …

Following the previous article, here we describe the first field demonstration of the ELVIS
system, performed at Newport Beach, CA. We examined ocean water to detect
microorganisms using the combined holographic and light-field fluorescence microscope
and successfully detected both eukaryotes and prokaryotes. The shared field of view
provided simultaneous bright-field (amplitude), phase, and fluorescence information from
both chlorophyll autofluorescence and acridine orange staining. The entire process was …