Tracking the Endosomal Escape of Nanoparticles in Live Cells Using a Triplex‐Forming Oligonucleotide

SK Bhangu, L Mummolo, S Fernandes… - Advanced Functional …, 2024 - Wiley Online Library
… to polyplexes and lipoplexes following endosomal escape. [ 5 ] … curves at different pH and
low ionic strength (14 mm NaCl, … by both contact and FRET effects (ie, FRET triplex = 100%) a …

Molecular beacon strategies for sensing purpose

N Bidar, M Amini, F Oroojalian, B Baradaran… - TrAC Trends in …, 2021 - Elsevier
… Pb 2+ ions in biological samples such as human serum samples and water with … existence
of K + , the sensor created a firm G-quadruplex with the two dyes in near proximity to let FRET

Mechanistic Study of DNA Delivery from Self-Assembled Nanolayered Films

S Wang - 2022 - dspace.mit.edu
… Solution pH and ionic strength-dependent changes in film … nucleases that degrade DNA,
serum proteins that mark DNA for … interactions resulting in lipoplexes with net positive charge, …

Inclusion of TAT and NLS sequences in lipopeptide molecules generates homogenous nanoparticles for gene delivery applications

S Pambudi, C Sriherwanto, AN Sasangka… - International Journal of …, 2024 - Elsevier
… The presence of 15 positive … ionic strength of the transfection agent/DNA certainly affect
complex stability. In addition to influence the particle size and complex the stability, ionic strength

Structure-Activity Relationships in the Biological Interactions of Spherical Nucleic Acids

G Yamankurt - 2020 - search.proquest.com
… design features affect biological activity, structure-activity … SNAs are recognized by different
serum nucleases than linear … in the salt aging step, 3) presence of a 3-hydroxypropyl disulfide …

Methods for intracellular delivery of quantum dots

SO Souza, RB Lira, CRA Cunha, BS Santos… - Topics in Current …, 2021 - Springer
… for specific labeling of intracellular structures. Over the years, … Cy5 resulted in high FRET,
whereas lipoplex destabilization, … These authors electroporated fibroblasts in the presence of …

Design and evaluation of drug-aptamer assemblies for targeted delivery to infectious and cancer diseases

M Cao - 2023 - theses.hal.science
… des médicaments et les cibler au site d’action. Par rapport à la littérature existante, ce travail
… can be condensed into spherical structures in the presence of magnesium ions95. Upon …

Oligonucleotide probes for imaging and diagnosis of bacterial infections

L Moreira, NM Guimarães, RS Santos… - Critical Reviews in …, 2024 - Taylor & Francis
… and is relatively insensitive to ionic strength due to the overall … One potential disadvantage
of using FRET and QUAL linear … to proteolytic degradation in human serum and cells [Citation…

Hydrophilic hyperbranched polymer-coated siRNA/polyamidoamine dendron-bearing lipid complexes preparation for high colloidal stability and efficient RNAi

E Yuba, T Korenaga, A Harada - Bioconjugate Chemistry, 2021 - ACS Publications
… of siRNA into target cells, serum resistance, and colloidal … Next, interaction of lipoplexes
with cells and the RNAi effect … Fluorescence resonance energy transfer (FRET) analysis was …

Synthesis and Characterization of Two Pyrene Labeled Gemini Surfactants and their Interactions with Oppositely Charged Molecules

A Ba Salem - 2022 - uwspace.uwaterloo.ca
… of fluorescence to probe the lipoplex/polyplex generated from the … Since the ionic strength of
the solution affects the CMC of … carriers and scaffolds for FRET measurements, as spacers to …