… of humaninduced pluripotent stem cells and mesenchymal stem cell differentiation potential to insulin producing cells in 2D and 3D culture systems in vitro

MF Abazari, N Nasiri, F Nejati… - Journal of Cellular …, 2020 - Wiley Online Library
… The transplantation of MSCs into each of the tissues that originates from the ectoderm,
endoderm and mesoderm causes the cells to differentiate into the target tissue. The iPSCs have …

Enhanced differentiation of human pluripotent stem cells into pancreatic endocrine cells in 3D culture by inhibition of focal adhesion kinase

X Liu, J Qin, M Chang, S Wang, Y Li, X Pei… - Stem Cell Research & …, 2020 - Springer
… of insulin-producing cells from human pluripotent stem cells (… are differentiated through the
stages of definitive endoderm (… to 3D culture for differentiation into EPs-3D and PECs-3D. b–i …

Continuous Inhibition of Sonic Hedgehog Signaling Leads to Differentiation of HumanInduced Pluripotent Stem Cells into Functional InsulinProducing β Cells

S Lee, JH Joo, JY Oh, EH Seo, YH Kim… - Stem Cells …, 2021 - Wiley Online Library
… the differentiation of cells into a definitive endoderm and … of a total induction period of 3 d.
Immunostaining revealed that … similarly induced differentiation into IPCs over 8 days in culture

[PDF][PDF] Developments in endoderm and pancreatic β-cell differentiation from human pluripotent stem cells

DH Lee, H Choo, HY Choi, SH Lee - 2024 - j-organoid.org
… Generating insulin-producing functional pancreatic β cells from … tent ability to self-renew
and differentiate while in culture [… Induced human pluripotent stem cells (hiPSCs) offer nearly …

Inhibition of LSD1 promotes the differentiation of human induced pluripotent stem cells into insulin-producing cells

XF Yang, SY Zhou, C Wang, W Huang, N Li… - Stem Cell Research & …, 2020 - Springer
… self-renewal and differentiation. We propose a new strategy to acquire insulin-producing cells
(… the loss of LSD1 may activate SOX17 and promote hiPSCs to differentiate into endoderm. …

Electrospun silk nanofibers improve differentiation potential of human induced pluripotent stem cells to insulin producing cells

SE Enderami, SF Ahmadi, RN Mansour… - Materials Science and …, 2020 - Elsevier
endoderm markers like FoxA2 and Sox-17 were measured by quantitative PCR. The maximum
levels of definitive endoderm markers were detected in the 3D … , using 3D culture system …

Stem cells differentiation into insulin-producing cells (IPCs): recent advances and current challenges

IBB Silva, CH Kimura, VP Colantoni… - Stem cell research & …, 2022 - Springer
… β-cell cultures in vitro to increase the supply of these cells for … control the differentiation of
induced pluripotent stem cells (… parameters for efficient endoderm differentiation is definition …

Generation of high yield insulin-producing cells (IPCs) from various sources of stem cells

A Soltani, S Khazaei, SM Mirtaghi, SF Ahmadi… - Vitamins and …, 2021 - Elsevier
induced pluripotent stem cells (iPSCs) are a group of cells … A and Wnt3 is used to definitive
endoderm generation. As in the … of the 3D media culture on the survival and differentiation of …

Optimization of 3D islet-like cluster derived from human pluripotent stem cells: An efficient in vitro differentiation protocol

S Ghorbani-Dalini, N Azarpira, MH Sangtarash… - Gene, 2022 - Elsevier
… into 3D glucose-responsive insulin-producing islet-like cluster. … Adherent culture refers to
S1-S4 while suspension culture … step, the decreased level of endoderm markers in our islet-like …

Generation of insulin-producing pancreatic β cells from multiple human stem cell lines

NJ Hogrebe, KG Maxwell, P Augsornworawat… - Nature protocols, 2021 - nature.com
differentiation methodology for generating human pluripotent … added during the subsequent
3 d of endoderm induction. At … for HUES8 and two induced pluripotent stem cell (iPSC) lines …