Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
… Analytical tools for fluorescence lifetime estimations, image segmentation, and heterogeneity
analysis are introduced in this section. Many popular methods are highlighted and …

Light-field tomographic fluorescence lifetime imaging microscopy

Y Ma, J Park, L Huang, C Sen, S Burri… - Proceedings of the …, 2024 - pnas.org
… significant spectral overlaps but differ in fluorescence lifetimes. The combination of four …
for conventional imaging techniques. Specifically, neither FLIM nor spectral imaging alone can …

Spatial resolution improved fluorescence lifetime imaging via deep learning

D Xiao, Z Zang, W Xie, N Sapermsap, Y Chen… - Optics …, 2022 - opg.optica.org
… to rapidly obtain HR FLIM images from raw image data … method combines DL-based
fluorescence lifetime determination and single image resolution enhancement (DL-SIRE) techniques

FLImBrush: dynamic visualization of intraoperative free-hand fiber-based fluorescence lifetime imaging

M Marsden, T Fukazawa, YC Deng… - Biomedical Optics …, 2020 - opg.optica.org
… Here we report FLImBrush as a robust method for the … fluorescence lifetime imaging (FLIm).
FLImBrush builds upon an existing method while employing deep learning-based image

Phasor S-FLIM: a new paradigm for fast and robust spectral fluorescence lifetime imaging

L Scipioni, A Rossetta, G Tedeschi, E Gratton - Nature methods, 2021 - nature.com
… of fluorescent specimens at optical resolution. To show the flexibility of the Phasor S-FLIM
technology and its applications … blind unmixing of spectral and lifetime signatures from multiple …

Real-time pixelwise phasor analysis for video-rate two-photon fluorescence lifetime imaging microscopy

JE Sorrells, RR Iyer, L Yang, AJ Bower… - Biomedical optics …, 2021 - opg.optica.org
Fluorescence lifetime imaging microscopy (FLIM) is an optical imaging technique that
characterizes the fluorescence profile of each pixel within an image by the average time between …

Fast wide-field upconversion luminescence lifetime thermometry enabled by single-shot compressed ultrahigh-speed imaging

X Liu, A Skripka, Y Lai, C Jiang, J Liu, F Vetrone… - Nature …, 2021 - nature.com
… Photoluminescence lifetime imaging of upconverting … of wide-field photoluminescence
lifetime imaging in real time, thus … -shot photoluminescence lifetime imaging thermometry (SPLIT). …

Histogram clustering for rapid time-domain fluorescence lifetime image analysis

Y Li, N Sapermsap, J Yu, J Tian, Y Chen… - Biomedical Optics …, 2021 - opg.optica.org
… We propose a histogram clustering (HC) method to accelerate fluorescence lifetime imaging
(FLIM) analysis in pixel-wise and global fitting modes. The proposed method’s principle was …

Label-free sensing of cells with fluorescence lifetime imaging: The quest for metabolic heterogeneity

EA Shirshin, MV Shirmanova… - Proceedings of the …, 2022 - National Acad Sciences
… Here, we demonstrate that fluorescence lifetime imaging with the advanced detection and
signal analysis system may allow rapid, sensitive, and noninvasive assessment of cellular …

The phasor plot: a universal circle to advance fluorescence lifetime analysis and interpretation

L Malacrida, S Ranjit, DM Jameson… - Annual Review of …, 2021 - annualreviews.org
… to fluorescence lifetime imaging has become a common method to analyze complicated
fluorescence … The appeal of the phasor representation of complex fluorescence decays in …