Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications
… Analytical tools for fluorescence lifetime estimations, image segmentation, and heterogeneity
analysis are introduced in this section. Many popular methods are highlighted and …
analysis are introduced in this section. Many popular methods are highlighted and …
Light-field tomographic fluorescence lifetime imaging microscopy
… significant spectral overlaps but differ in fluorescence lifetimes. The combination of four …
for conventional imaging techniques. Specifically, neither FLIM nor spectral imaging alone can …
for conventional imaging techniques. Specifically, neither FLIM nor spectral imaging alone can …
Spatial resolution improved fluorescence lifetime imaging via deep learning
… to rapidly obtain HR FLIM images from raw image data … method combines DL-based
fluorescence lifetime determination and single image resolution enhancement (DL-SIRE) techniques…
fluorescence lifetime determination and single image resolution enhancement (DL-SIRE) techniques…
FLImBrush: dynamic visualization of intraoperative free-hand fiber-based fluorescence lifetime imaging
… Here we report FLImBrush as a robust method for the … fluorescence lifetime imaging (FLIm).
FLImBrush builds upon an existing method while employing deep learning-based image …
FLImBrush builds upon an existing method while employing deep learning-based image …
Phasor S-FLIM: a new paradigm for fast and robust spectral fluorescence lifetime imaging
… of fluorescent specimens at optical resolution. To show the flexibility of the Phasor S-FLIM
technology and its applications … blind unmixing of spectral and lifetime signatures from multiple …
technology and its applications … blind unmixing of spectral and lifetime signatures from multiple …
Real-time pixelwise phasor analysis for video-rate two-photon fluorescence lifetime imaging microscopy
… Fluorescence lifetime imaging microscopy (FLIM) is an optical imaging technique that
characterizes the fluorescence profile of each pixel within an image by the average time between …
characterizes the fluorescence profile of each pixel within an image by the average time between …
Fast wide-field upconversion luminescence lifetime thermometry enabled by single-shot compressed ultrahigh-speed imaging
… Photoluminescence lifetime imaging of upconverting … of wide-field photoluminescence
lifetime imaging in real time, thus … -shot photoluminescence lifetime imaging thermometry (SPLIT). …
lifetime imaging in real time, thus … -shot photoluminescence lifetime imaging thermometry (SPLIT). …
Histogram clustering for rapid time-domain fluorescence lifetime image analysis
… We propose a histogram clustering (HC) method to accelerate fluorescence lifetime imaging
(FLIM) analysis in pixel-wise and global fitting modes. The proposed method’s principle was …
(FLIM) analysis in pixel-wise and global fitting modes. The proposed method’s principle was …
Label-free sensing of cells with fluorescence lifetime imaging: The quest for metabolic heterogeneity
EA Shirshin, MV Shirmanova… - Proceedings of the …, 2022 - National Acad Sciences
… Here, we demonstrate that fluorescence lifetime imaging with the advanced detection and
signal analysis system may allow rapid, sensitive, and noninvasive assessment of cellular …
signal analysis system may allow rapid, sensitive, and noninvasive assessment of cellular …
The phasor plot: a universal circle to advance fluorescence lifetime analysis and interpretation
… to fluorescence lifetime imaging has become a common method to analyze complicated
fluorescence … The appeal of the phasor representation of complex fluorescence decays in …
fluorescence … The appeal of the phasor representation of complex fluorescence decays in …
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