[HTML][HTML] Plasmid transfer by conjugation in Gram-negative bacteria: from the cellular to the community level

C Virolle, K Goldlust, S Djermoun, S Bigot, C Lesterlin - Genes, 2020 - mdpi.com
… regulation of the plasmid tra genes required for conjugation, the processing of the … initiation
sequence, are found on various conjugative plasmids, including R6K, R100, ColE1, ColE2, …

Growth‐stage‐dependent regulation of conjugation

TA Sysoeva, Y Kim, J Rodriguez, AJ Lopatkin… - AIChE …, 2020 - Wiley Online Library
… , which consists of genes required for the expression of DNA processing proteins (relaxase), …
of the conjugative plasmids R6K and pOLA52, we designed primers specific to the cpl gene

Refactoring the conjugation machinery of promiscuous plasmid RP4 into a device for conversion of gram-negative isolates to Hfr strains

J Silbert, V Lorenzo, T Aparicio - ACS Synthetic Biology, 2021 - ACS Publications
included a mini-Tn5 transposon (Km R ) loaded with the Tra1-Tra2 gene cluster and also a
suicidal R6K plasmid … the media composition) on the conjugation and DNA transfer process. …

[HTML][HTML] Cobalt complexes modulate plasmid conjugation in Escherichia coli and Klebsiella pneumoniae

I Alav, P Pordelkhaki, PE de Resende, H Partington… - Scientific Reports, 2024 - nature.com
… as a recipient for conjugation assays, the hph gene encoding … the conjugation process
rather than plasmid persistence in … plasmid R6K, only Co4 significantly reduced its conjugation

[HTML][HTML] Highly efficient gene transfer in the mouse gut microbiota is enabled by the Incl2 conjugative plasmid TP114

K Neil, N Allard, F Grenier, V Burrus… - Communications …, 2020 - nature.com
… microbial species to exchange genes and adapt to various … is bacterial conjugation, a natural
process during which DNA is … other plasmids (R64, pIP72, RIP71a, R1, pOX38, and R6K) …

Origins of transfer establish networks of functional dependencies for plasmid transfer by conjugation

M Ares-Arroyo, C Coluzzi, EPC Rocha - Nucleic acids research, 2023 - academic.oup.com
… with many plasmids, whereas conjugative plasmids are the … characterized plasmid mobility
in relation to conjugation and … the plasmids in terms of the protein-coding genes involved in …

[HTML][HTML] Alpha-Helical Protein KfrC Acts as a Switch between the Lateral and Vertical Modes of Dissemination of Broad-Host-Range RA3 Plasmid from IncU (IncP-6) …

M Mitura, E Lewicka, J Godziszewska… - International Journal of …, 2021 - mdpi.com
… KfrA were involved in the plasmid maintenance and conjugative … the clusters of functionally
related genes, designated the … Previously, we showed that the conjugative transfer process of …

Conjugation dynamics depend on both the plasmid acquisition cost and the fitness cost

H Prensky, A Gomez‐Simmonds… - Molecular systems …, 2021 - embopress.org
… The absence of genes encoding for conjugation machinery … in R1 or R6K acquisition costs,
regardless of conjugation … acquisition and subsequent adaptation is a continuous process, we …

[HTML][HTML] Dominance between plasmids determines the extent of biofilm formation

JA Gama, EGA Fredheim, F Cléon, AM Reis… - Frontiers in …, 2020 - frontiersin.org
… surface exclusion (a process by which plasmid-carrying cells … We show that the ability of
conjugative plasmids to promote … the IncX2 plasmid R6K is related to IncX1 plasmids, we did not …

PixR, a Novel Activator of Conjugative Transfer of IncX4 Resistance Plasmids, Mitigates the Fitness Cost of mcr-1 Carriage in Escherichia coli

L Yi, R Durand, F Grenier, J Yang, K Yu, V Burrus… - MBio, 2022 - Am Soc Microbiol
… similarity with the IncX family reference plasmid R6K. … TaxB, which are involved in DNA
processing during conjugation (… for genes involved in the conjugative transfer of IncX4 plasmids. …