… The third method for rAAV transient production is using a recombinant Herpes Simplex
virus (rHSV), which is a replication-deficient viral vector, to infect mammalian cells such as …

… a high titer producing clone remains one of the major drawbacks. This thesis aimed to overcome
these issues by using … helper viruses, such as Adenovirus or Herpes Simplex Virus, are …

… Recombinant adeno-associated viruses (rAAVs) are robust and versatile tools for … using
gradients to purify rAAVs are time consuming and requires multiple purification steps to produce …

… vectors such as the adenovirus, herpes simplex virus (HSV), or the baculovirus as carriers of
… Using GX6A and GX6B, we screened for induction conditions that could produce high-titer …

… -free, helper virus-free rAAV producer cell line using synthetic … Herpes simplex virus (HSV)
was identified as an alternative … , high-titer 293 cell-based adeno-associated virus packaging …

… viruses, adenovirus type 5 (AdV5) and herpes simplex virus … successfully been utilized for
AAV vector production in the … replication and hence, the production of AAV progeny, consists of …

… validation was conducted using HEK293s for generality. rAAV2 production was enhanced
… OneBac: platform for scalable and high-titer production of adeno-associated virus serotype 1…

… viruses can be difficult to cultivate to high titer in cells that … strategy is often utilized by other
viruses to evade antiviral … , ICP0 is a viral protein expressed by the herpes simplex virus 1 (…

… plasmid transfection or viral transduction utilizing well-… by other pathogenic viruses, such as
herpes simplex virus (HSV) … Rep proteins, yet allow high titer production by a novel laboratory…

… Herpes simplex virus (HSV) complementation platforms are another alternative for AAV …
that they require production of two distinct HSV vectors to high titer prior to using them for making …