H2A mono-ubiquitination differentiates FACT's functions in nucleosome assembly and disassembly
YZ Wang, C Liu, J Zhao, J Yu, A Luo, X Xiao… - Nucleic acids …, 2022 - academic.oup.com
… histone octamers, tetramers and dimers were reconstituted … tethers the histones to the DNA
and allows the reassembly of H2A … –H2B dimers onto the (H3–H4) 2 tetrasome (the particles …
and allows the reassembly of H2A … –H2B dimers onto the (H3–H4) 2 tetrasome (the particles …
Rapid reconstitution of ubiquitinated nucleosome using a non-denatured histone octamer ubiquitylation approach
W Li, P Cao, P Xu, F Sun, C Wang, J Zhang, S Dong… - Cell & Bioscience, 2024 - Springer
… of ubiquitin to specific lysine sites on H2A/H2B tails on histone … enough to permit modifications
at various histone sites and … is stimulated by histone H2B Lys120 ubiquitination [35, 41,42,…
at various histone sites and … is stimulated by histone H2B Lys120 ubiquitination [35, 41,42,…
Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch
… Such flexibility could, in principle, allow ubiquitin to adopt … We note that iterative Ab-Initio
Reconstruction jobs for particle … To see whether ubiquitin conjugated to H2A-K119 adopts …
Reconstruction jobs for particle … To see whether ubiquitin conjugated to H2A-K119 adopts …
Decoding histone ubiquitylation
JJ Chen, D Stermer, JC Tanny - Frontiers in Cell and Developmental …, 2022 - frontiersin.org
… Moreover, a reconstituted PRC2 complex containing both … a solvent-exposed cleft between
histones H2B and H4. The C… then makes additional stabilizing contacts with the H2A/H2B …
histones H2B and H4. The C… then makes additional stabilizing contacts with the H2A/H2B …
Histone H2A ubiquitination reinforces mechanical stability and asymmetry at the single-nucleosome level
X Xiao, C Liu, Y Pei, YZ Wang, J Kong… - Journal of the …, 2020 - ACS Publications
… ) or unmodified H2A (H2A-nucleosome) were reconstituted in vitro … ubH2A gives rise to
different folding kinetics as well as … were similar to that of normal H2A-nucleosome (Figure 3d), …
different folding kinetics as well as … were similar to that of normal H2A-nucleosome (Figure 3d), …
H2B ubiquitination recruits FACT to maintain a stable altered nucleosome state for transcriptional activation
A Luo, J Kong, J Chen, X Xiao, J Lan, X Li, C Liu… - Nature …, 2023 - nature.com
… obtained by ubH2B and FACT provides a key platform for gene … Histone H2B is transiently
ubiquitinated and deubiquitinated at … To reconstitute the respective histone octamers, H3/H4 …
ubiquitinated and deubiquitinated at … To reconstitute the respective histone octamers, H3/H4 …
The Effects of Histone H2B Ubiquitylations on the Nucleosome Structure and Internucleosomal Interactions
… structure of a nucleosome core particle reconstituted with the Widom … the ubiquitin
molecules in H2BK34ub nucleosomes protrude between the DNA gyres and relax the DNA–(H2A–H2B…
molecules in H2BK34ub nucleosomes protrude between the DNA gyres and relax the DNA–(H2A–H2B…
The intrinsic stability of H2B-ubiquitylated nucleosomes and their in vitro assembly/disassembly by histone chaperone NAP1
WA Krajewski - Biochimica et Biophysica Acta (BBA)-General Subjects, 2020 - Elsevier
… Nucleosomes were reconstituted by sequential dilutions … PAGE gives little advantages.
These markers do not … with ubiquitylated and unmodified histones H2B (what is probably more …
These markers do not … with ubiquitylated and unmodified histones H2B (what is probably more …
Structural basis of nucleosome dynamics modulation by histone variants H2A. B and H2A. Z. 2.2
M Zhou, L Dai, C Li, L Shi, Y Huang, Z Guo, F Wu… - The EMBO …, 2021 - embopress.org
… provides insights into the structure and function of the H2A.B‐… increase the reconstitution
efficiency and overall yield of H2A.… Histones H2B in H2A.B‐NCP are colored in warmpink, Their …
efficiency and overall yield of H2A.… Histones H2B in H2A.B‐NCP are colored in warmpink, Their …
[PDF][PDF] BIOCHEMICAL AND STRUCTURAL STUDIES OF THE ROLES OF FACT AND UBP10 IN HISTONE H2B DEUBIQUITINATION AND NUCLEOSOME DYNAMICS
ML Nune - 2021 - jscholarship.library.jhu.edu
… Ubp10 makes several contacts with histones H2A and H3 at the nucleosome dyad, with the
ubiquitin… This H2B-Ub was reconstituted with histone H2A to form H2A/H2B-Ub heterodimers. …
ubiquitin… This H2B-Ub was reconstituted with histone H2A to form H2A/H2B-Ub heterodimers. …