H2A mono-ubiquitination differentiates FACT's functions in nucleosome assembly and disassembly

YZ Wang, C Liu, J Zhao, J Yu, A Luo, X Xiao… - Nucleic acids …, 2022 - academic.oup.com
histone octamers, tetramers and dimers were reconstituted … tethers the histones to the DNA
and allows the reassembly of H2A … –H2B dimers onto the (H3–H4) 2 tetrasome (the particles

Rapid reconstitution of ubiquitinated nucleosome using a non-denatured histone octamer ubiquitylation approach

W Li, P Cao, P Xu, F Sun, C Wang, J Zhang, S Dong… - Cell & Bioscience, 2024 - Springer
… of ubiquitin to specific lysine sites on H2A/H2B tails on histone … enough to permit modifications
at various histone sites and … is stimulated by histone H2B Lys120 ubiquitination [35, 41,42,…

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch

CW Hicks, S Rahman, SL Gloor, JK Fields… - Nucleic Acids …, 2024 - academic.oup.com
… Such flexibility could, in principle, allow ubiquitin to adopt … We note that iterative Ab-Initio
Reconstruction jobs for particle … To see whether ubiquitin conjugated to H2A-K119 adopts …

Decoding histone ubiquitylation

JJ Chen, D Stermer, JC Tanny - Frontiers in Cell and Developmental …, 2022 - frontiersin.org
… Moreover, a reconstituted PRC2 complex containing both … a solvent-exposed cleft between
histones H2B and H4. The C… then makes additional stabilizing contacts with the H2A/H2B

Histone H2A ubiquitination reinforces mechanical stability and asymmetry at the single-nucleosome level

X Xiao, C Liu, Y Pei, YZ Wang, J Kong… - Journal of the …, 2020 - ACS Publications
… ) or unmodified H2A (H2A-nucleosome) were reconstituted in vitro … ubH2A gives rise to
different folding kinetics as well as … were similar to that of normal H2A-nucleosome (Figure 3d), …

H2B ubiquitination recruits FACT to maintain a stable altered nucleosome state for transcriptional activation

A Luo, J Kong, J Chen, X Xiao, J Lan, X Li, C Liu… - Nature …, 2023 - nature.com
… obtained by ubH2B and FACT provides a key platform for gene … Histone H2B is transiently
ubiquitinated and deubiquitinated at … To reconstitute the respective histone octamers, H3/H4 …

The Effects of Histone H2B Ubiquitylations on the Nucleosome Structure and Internucleosomal Interactions

B Sengupta, M Huynh, CB Smith, RK McGinty… - Biochemistry, 2022 - ACS Publications
… structure of a nucleosome core particle reconstituted with the Widom … the ubiquitin
molecules in H2BK34ub nucleosomes protrude between the DNA gyres and relax the DNA–(H2AH2B

The intrinsic stability of H2B-ubiquitylated nucleosomes and their in vitro assembly/disassembly by histone chaperone NAP1

WA Krajewski - Biochimica et Biophysica Acta (BBA)-General Subjects, 2020 - Elsevier
… Nucleosomes were reconstituted by sequential dilutions … PAGE gives little advantages.
These markers do not … with ubiquitylated and unmodified histones H2B (what is probably more …

Structural basis of nucleosome dynamics modulation by histone variants H2A. B and H2A. Z. 2.2

M Zhou, L Dai, C Li, L Shi, Y Huang, Z Guo, F Wu… - The EMBO …, 2021 - embopress.org
provides insights into the structure and function of the H2A.B‐… increase the reconstitution
efficiency and overall yield of H2A.… Histones H2B in H2A.B‐NCP are colored in warmpink, Their …

[PDF][PDF] BIOCHEMICAL AND STRUCTURAL STUDIES OF THE ROLES OF FACT AND UBP10 IN HISTONE H2B DEUBIQUITINATION AND NUCLEOSOME DYNAMICS

ML Nune - 2021 - jscholarship.library.jhu.edu
… Ubp10 makes several contacts with histones H2A and H3 at the nucleosome dyad, with the
ubiquitin… This H2B-Ub was reconstituted with histone H2A to form H2A/H2B-Ub heterodimers. …