Is plastidic glutamine synthetase essential for C3 plants? A tale of photorespiratory mutants, ammonium tolerance and conifers

D Marino, RA Cañas, M Betti - New Phytologist, 2022 - Wiley Online Library
… The glutamine synthetase/glutamate synthetase (GS/GOGAT) … Glutamine synthetase catalyzes
the conversion of glutamate (… mutants under ammonium nutrition, and this finding, together …

… GLUTAMINE SYNTHETASE 1;2 maintains carbon and nitrogen balance under high-ammonium conditions and results in increased tolerance to ammonium toxicity in …

X Leng, H Wang, L Cao, R Chang… - Journal of …, 2024 - academic.oup.com
… lines overexpressing GLUTAMINE SYNTHETASE 1;2 (GS1… determined. In addition, the
contents of various metabolites and elements, and activities of various enzymes were determined

[HTML][HTML] Excessive ammonium assimilation by plastidic glutamine synthetase causes ammonium toxicity in Arabidopsis thaliana

T Hachiya, J Inaba, M Wakazaki, M Sato… - Nature …, 2021 - nature.com
… be determined. Here, we show that ammonium assimilation by GLUTAMINE SYNTHETASE
… To ensure that reduced expression of GLN2 enhances ammonium tolerance, we obtained …

[HTML][HTML] Root GS and NADH-GDH play important roles in enhancing the ammonium tolerance in three bedding plants

J Song, J Yang, BR Jeong - International Journal of Molecular Sciences, 2022 - mdpi.com
… We determined that they possessed distinct ammonium … by glutamate synthetase (GOGAT)
to synthesize glutamate; … NH 4 + can also be transformed into glutamine by glutamate

[HTML][HTML] Nitrogen Regulating the Expression and Localization of Four Glutamine Synthetase Isoforms in Wheat (Triticum aestivum L.)

Y Wei, X Wang, Z Zhang, S Xiong, X Meng… - International Journal of …, 2020 - mdpi.com
… Our interesting finding was that the activity of TaGS1;2 in the reaction mixture with 60 mM …
NH 4 + concentration, plants will accumulate a large amount of ammonium [44] and maintain …

Glutamine synthetase: an unlikely case of functional redundancy in Arabidopsis thaliana

E Moreira, S Coimbra, P Melo - Plant Biology, 2022 - Wiley Online Library
… ;2 plants these symptoms appear also on mid-to-older leaves, confirming that Gln1;2 codes
for an isoenzyme that provides higher resistance to ammonium toxicity in Arabidopsis plants. …

[HTML][HTML] Ammonium phytotoxicity and tolerance: An insight into ammonium nutrition to improve crop productivity

J Shilpha, J Song, BR Jeong - Agronomy, 2023 - mdpi.com
glutamate synthase) and GS1 are implicated in nitrogen assimilation in the roots, while Fd-GOGAT
(reduced ferredoxin-dependent glutamate synthase) and GS2 are … been determined

[HTML][HTML] Integrative transcriptomic and proteomic analysis reveals an alternative molecular network of glutamine synthetase 2 corresponding to nitrogen deficiency in …

T Liang, Z Yuan, L Fu, M Zhu, X Luo, W Xu… - International Journal of …, 2021 - mdpi.com
… , glutamine synthetase (GS2), a chloroplastic ammonium … supported by GS enzyme activity
and glutamine/glutamate (Gln/… adapt the omics profiling to determine the morphological and …

… identification of molecular mechanism in which the overexpression of wheat cytosolic and plastid glutamine synthetases in tobacco enhanced drought tolerance

H Yu, Y Zhang, Z Zhang, J Zhang, Y Wei, X Jia… - Plant physiology and …, 2020 - Elsevier
… In higher plants, ammonium is mainly assimilated into Gln by GS (EC 6.3.1.2). The main plant
GS species is GSⅡ, which can … A t-test was applied to calculate fold change (FC) and false …

Physiologic, metabolomic, and genomic investigations reveal distinct glutamine and mannose metabolism responses to ammonium toxicity in allotetraploid rapeseed …

T Zhou, Y Hua, C Yue, J Huang, Z Zhang - Plant Science, 2021 - Elsevier
… NH 4 + uptake assays, glutamine synthetase (GS) activity … that the significantly higher GS
activity observed in T5 roots … and to determine the key metabolites involved in NH 4 + tolerance, …