The Structural Ensemble of the Conjugative F-like T4SS

N Bragagnolo, C Rodriguez… - … , Physiology, and …, 2021 - api.taylorfrancis.com
… these three proteins, with disulfide bond formation/cleavage likely … assembly of the relaxosome
enabling the rapid spread of … It is presumed that TraY and IHF induce a bend in DNA by 50…

Protein transfer through an F plasmid-encoded type IV secretion system suppresses the mating-induced SOS response

AAM Al Mamun, K Kishida, PJ Christie - MBio, 2021 - Am Soc Microbiol
… in a phosphodiesterase cleavage reaction that covalently tethers the … pED208 relaxosome
assembly and engagement with the TraD … Our findings suggest that pED208 relaxosome-TraD …

[HTML][HTML] Plasmid transfer by conjugation in Gram-negative bacteria: from the cellular to the community level

C Virolle, K Goldlust, S Djermoun, S Bigot, C Lesterlin - Genes, 2020 - mdpi.com
assembly and activity of a protein complex, the relaxosome, … (IHF) and the plasmid-encoded
TraY and TraM proteins, … filament, which stimulates the autocatalytic cleavage of LexA, the …

[PDF][PDF] Insights into key steps of DNA transfer and the host spectrum of the large conjugative plasmid pXO16 from Bacillus thuringiensis sv. israelensis

P HOLS - dial.uclouvain.be
… for the F plasmid and the R388 plasmid from Escherichia coli … -encoded IHF (integrated host
factor) form the relaxosome … mode: pilus assembly and DNA/protein secretion. The pilus is …

Bacteria DNA conjugation: from the cellular to the community level

C Virolle, K Goldlust, S Djermoun, S Bigot, C Lesterlin - 2020 - preprints.org
assembly and activity of a protein complex, the relaxosome, … factor IHF and the plasmid-encoded
TraY and TraM proteins, … filament, which stimulates the autocatalytic cleavage of LexA, …

IncFV plasmid pED208: Sequence analysis and evidence for translocation of maintenance/leading region proteins through diverse type IV secretion systems

AAM Al Mamun, K Kissoon, K Kishida, WC Shropshire… - Plasmid, 2022 - Elsevier
… for assembly of R388 pili. Various features of the T4SS … two proteins termed PsiA and PsiB
for plasmid-SOS-inhibitors B … requires assembly of the pED208 relaxosome and plasmid co-…

Structural and functional diversity of type IV secretion systems

TRD Costa, JB Patkowski, K Macé, PJ Christie… - Nature Reviews …, 2024 - nature.com
… , the relaxosome is composed of four proteins assembled at … Two other relaxosome
components, TraY and IHF, cause … DNA topology that expose the nick (nic) site for cleavage by …

[HTML][HTML] Protein dynamics in F-like bacterial conjugation

N Bragagnolo, C Rodriguez, N Samari-Kermani… - Biomedicines, 2020 - mdpi.com
that TraE may contain a leader sequence that is cleaved prior … assembly of the relaxosome,
allowing for the rapid spread of … The assembly of both TraY and IHF at oriT is required prior to …

Study of bacterial conjugation from a practical and fundamental perspective

A Reuter - 2021 - theses.hal.science
… The relaxosome is a protein complex assembling at a … 3’ end generated by the cleavage
reaction serves to initiate the … IncX R485 plasmids respectively to inhibit pilus assembly of IncF …

[HTML][HTML] Secrete or perish: the role of secretion systems in Xanthomonas biology

CE Alvarez-Martinez, GG Sgro, GG Araujo… - Computational and …, 2021 - Elsevier
… is believed to aid the assembly of the extracellular appendages … also carry mobilizable
plasmids that code for separate … ) cleavage site, multiple C-terminal RTX motifs, and are secreted …