… were diluted with cell culture medium, and 1 × 10 4 cells/well/… and ERβ inhibitors led to the
determination that ERα, not ERβ… that estrogen supplementation may be a potential approach …

… After identifying the potential risks of bias in current in vitro … For isolated stromal or epithelial
cell cultures, single stromal cells … , steroid hormone receptor expression or steroid hormone …

… of the nuclear hormone receptor superfamily. Several ERs have been identified: the nuclear
… niche [94], but the possible effects of E2 on bone marrow endothelial cells are still unknown. …

… is mediated by its interaction with estrogen receptor (ER)α … requires the identification of
possible targets and/or … the culture medium may have provided enough estrogen level …

… Our study is the first to explore (i) how estrogen receptor β (… using an ECL chemiluminescent
detection system (Thermo … Results from our co-culture of RCC cells with endothelial cells (…

… on endothelial cells, the main cellular target of estrogens. … Collectively, so far animal studies
have helped to identify at … cell culture is known to elicit a repression of ERα expression. …

… Identification of estrogen receptor binding sites located at the … Mouse endothelial cells were
isolated from aorta of WT and … These mouse endothelial cells obtained from primary cultures …

… cell culture supernatant from the same cell line and 20% FBS. Growing HEK-ESR1 cell clones
were further cultured … In addition, TP53 was also identified as possible upstream regulator. …

… mechanism to estrogen receptor-mediated hormone actions. … transformation in human
breast epithelial cells [13]. … However, they lack the efficacy of traditional estrogen. SERMs are …

… were analyzed by flow cytometry or traditional fluorescence … the normal cellular mitochondrial
membrane potential. The … Endothelial estrogen receptor- alpha plays a crucial role in …